UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 3 > Ahmed M. Abdellatif1,2)*, Kiyohito Ogata1)*, Takashi Kudo1)*, Gulibaikelamu Xiafukaiti1), Yu-Hsin Chang1), Megumi C. Katoh1), Salah E. El-Morsy2), Hisashi Oishi1,3), and Satoru Takahashi1,3,4)

Role of large MAF transcription factors in the mouse endocrine pancreas

Ahmed M. Abdellatif1,2)*, Kiyohito Ogata1)*, Takashi Kudo1)*, Gulibaikelamu Xiafukaiti1), Yu-Hsin Chang1), Megumi C. Katoh1), Salah E. El-Morsy2), Hisashi Oishi1,3), and Satoru Takahashi1,3,4)
1)Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan 2)Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Mansoura University, Elgomhouria Street, Mansoura, Dakahlyia 35516, Egypt 3)Life Science Center, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan 4)International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan
Abstract :

The members of the MAF family of transcription factors are homologs of v-Maf –the oncogenic component of the avian retrovirus AS42. The MAF family is subdivided into 2 groups, small and large MAFs. To elucidate the role of the large MAF transcription factors in the endocrine pancreas, we analyzed large MAF gene knockout mice. It has been shown that Mafa−/− mice develop phenotypes including abnormal islet structure soon after birth. This study revealed that Ins1 and Ins2 transcripts and the protein contents were significantly reduced in Mafa−/− mice at embryonic day 18.5. In addition, Mafa−/−;Mafb−/− mice contained less than 10% of the insulin transcript and protein of those of wildtype mice, suggesting that Mafa and Mafb cooperate to maintain insulin levels at the embryonic stage. On the other hand, the number of insulin-positive cells in Mafa−/− mice was comparable to that of wild-type mice, and even under a Mafb-deficient background the number of insulin-positive cells was not decreased, suggesting that Mafb plays a dominant role in embryonic β-cell development. We also found that at 20 weeks of age Mafa−/−;Mafb+/− mice showed a higher fasting blood glucose level than single Mafa−/− mice. In summary, our results indicate that Mafa is necessary for the maintenance of normal insulin levels even in embryos and that Mafb is important for the maintenance of fasting blood glucose levels in the Mafa-deficient background in adults.

Keywords :
diabetes mellitus, glucagon, insulin, large MAF, transcription factor

Date Deposited : 07 Jan 2016 10:45

Last Modified : 07 Jan 2016 10:45

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/3/_contents

Volume 64, Number 3, April 2015 , ISSN 305–312

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal