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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 4 > Hye Min Shim1), Ji Young Hwang1), Kyung Min Lee1), Yunhwa Kim1), Daewon Jeong1), Jaesook Roh2), Hyeonhae Choi2), Jung Hye Hwang3), and Hosun Park1)

Coxsackievirus B3 infection reduces female mouse fertility

Hye Min Shim1), Ji Young Hwang1), Kyung Min Lee1), Yunhwa Kim1), Daewon Jeong1), Jaesook Roh2), Hyeonhae Choi2), Jung Hye Hwang3), and Hosun Park1)
1)Department of Microbiology, College of Medicine, Yeungnam University, 170, Hyeonchung-ro, Namgu, Daegu 705-703, Republic of Korea 2)Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul 133-791, Republic of Korea 3)Department of Obstetrics and Gynecology, College of Medicine, Hanyang University Hospital, 222, Wangsimniro, Seongdong-gu, Seoul 133-791, Republic of Korea
Abstract :

Previously we demonstrated coxsackievirus B3 (CVB3) infection during early gestation as a cause of pregnancy loss. Here, we investigated the impacts of CVB3 infection on female mouse fertility. Coxsackievirus-adenovirus receptor (CAR) expression and CVB3 replication in the ovary were evaluated by immunohistochemistry or reverse transcription-polymerase chain reaction (RTPCR). CAR was highly expressed in granulosa cells (GCs) and CVB3 replicated in the ovary. Histological analysis showed a significant increase in the number of atretic follicles in the ovaries of CVB3-infected mice (CVBM). Estrous cycle evaluation demonstrated that a higher number of CVBM were in proestrus compared to mock mice (CVBM vs. mock; 61.5%, 28.5%, respectively). Estradiol concentration in GC culture supernatant and serum were measured by an enzyme-linked immunosorbent assay. Baseline and stimulated levels of estradiol in GC were decreased in CVBM, consistent with significantly reduced serum levels in these animals. In addition, aromatase transcript levels in GCs from CVBM were also decreased by 40% relative to the mock. Bone mineral density evaluated by micro-computed tomography was significantly decreased in the CVBM. Moreover, the fertility rate was also significantly decreased for the CVBM compared to the mock (CVBM vs. mock; 20%, 94.7%, respectively). This study suggests that CVB3 infection could interfere with reproduction by disturbing ovarian function and cyclic changes of the uterus.

Keywords :
coxsackievirus-adenovirus reseptor (CAR), coxsackievirus B3 (CVB3), estradiol, granulosa cell, infertility

Date Deposited : 07 Jan 2016 10:59

Last Modified : 07 Jan 2016 10:59

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/4/_contents

Volume 64, Number 4, June 2015 , ISSN 343–352

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