UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 4 > Yutaka Nakamura1), Atsushi Asano1), Yoshinao Hosaka2), Takashi Takeuchi3), Toshihiko Iwanaga4), and Yoshiaki Yamano1)

Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes

Yutaka Nakamura1), Atsushi Asano1), Yoshinao Hosaka2), Takashi Takeuchi3), Toshihiko Iwanaga4), and Yoshiaki Yamano1)
1)Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Japan 2)Laboratory of Veterinary Anatomy, Faculty of Agriculture, Tottori University, Japan 3)Laboratory of Laboratory Animal Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan 4)Laboratory of Histology and Cytology, Department of Functional Morphology, Graduate school of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan
Abstract :

Membrane trafficking in male germ cells contributes to their development via cell morphological changes and acrosome formation. TBC family proteins work as Rab GTPase accelerating proteins (GAPs), which negatively regulate Rab proteins, to mediate membrane trafficking. In this study, we analyzed the expression of a Rab GAP, TBC1D9, in mouse organs and the intracellular localization of the gene products. Tbc1d9 showed abundant expression in adult mice testis. We found that the Tbc1d9 mRNA was expressed in primary and secondary spermatocytes, and that the TBC1D9 protein was expressed in spermatocytes and round spermatids. In 293T cells, TBC1D9-GFP proteins were localized in the endosome and Golgi apparatus. Compartments that were positive for the constitutive active mutants of Rab7 and Rab9 were also positive for TBC1D9 isoform 1. In addition, TBC1D9 proteins were associated with Rab7 and Rab9, respectively. These results indicate that TBC1D9 is expressed mainly in spermatocytes, and suggest that TBC1D9 regulates membrane trafficking pathways related to Rab9- or Rab7-positive vesicles.

Keywords :
Rab7, Rab9, Spermatogenesis, TBC1D9

Date Deposited : 07 Jan 2016 11:30

Last Modified : 07 Jan 2016 11:30

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/4/_contents

Volume 64, Number 4, June 2015 , ISSN 415–424

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal