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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2015 > Volume 64 Number 4 > Zhang Zhuhua#, Wang Zhiquan#, Yang Zhen#, Niu Yixin, Zhang Weiwei, Li Xiaoyong, Liu Yueming, Zhang Hongmei, Qin Li, and Su Qing

A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice

Zhang Zhuhua#, Wang Zhiquan#, Yang Zhen#, Niu Yixin, Zhang Weiwei, Li Xiaoyong, Liu Yueming, Zhang Hongmei, Qin Li, and Su Qing
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Abstract :

Currently, the metabolic syndrome (MS) is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Male Institute of Cancer Research (ICR) mice were fed with high-fat-high-fructose (HFHF) diet or normal chow (NC) respectively for 12 weeks. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. Serum insulin levels were measured by ELISA, and lipid profiles were determined biochemically. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. Our data suggest that HFHF diet-fed ICR mice may be a robust and efficient animal model that could well mimic the basic pathogenesis of human MS.

Keywords :
animal model, high-fat-high-fructose, Institute of Cancer Research mice, insulin resistance, metabolic syndrome

Date Deposited : 07 Jan 2016 11:36

Last Modified : 07 Jan 2016 11:36

Official URL: http://https://www.jstage.jst.go.jp/browse/expanim/64/4/_contents

Volume 64, Number 4, July 2015 , ISSN 435–442

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