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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 2 > Kaori YABUUCHI1), Masaru TAJIMA1), Yuko KOTANI1), Katsumi AIHARA1), Aiko KAWASAKI1), Shiro KANEKO1), Yasuhiro TAKAGI1), Sumiko KAWAI1), Kyoko SHIOYA2), Akira OKAMOTO1), Soichiro KAGIYAMA1) and Tsutomu KUROSAWA1)

A Diagnostic Method for Pneumocystis carinii a Causative Agent of Pneumonia in Immunodeficient Rats

Kaori YABUUCHI1), Masaru TAJIMA1), Yuko KOTANI1), Katsumi AIHARA1), Aiko KAWASAKI1), Shiro KANEKO1), Yasuhiro TAKAGI1), Sumiko KAWAI1), Kyoko SHIOYA2), Akira OKAMOTO1), Soichiro KAGIYAMA1) and Tsutomu KUROSAWA1)
1) Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine 2) National Cardiovascular Center, Research Institute Laboratory Animal Unit
Abstract :

Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We could detect positive signals at identical locations with IC and Toluidine blue O in lungs of infected rats. These results corresponded to the results obtained with PCR. We should study the relationship between unexpected positive signals seen in IC and trophic forms in lungs of infected rats. We could clinically diagnose pneumonia caused by Pneumocystis carinii with the diagnostic method we developed.

Keywords :
immunochemistry, immunodeficient rat, Pneumocystis carinii

Date Deposited : 21 Jun 2011 14:09

Last Modified : 21 Jun 2011 14:15

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 2, - 2010

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