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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 4 > Hideharu OCHIAI1), Miyoko SAITO2), Takuya MARUO3) and Nobuyuki KANEMAKI3)

Molecular Cloning of Canine Excitatory Amino Acid Transporter 5 and Its Detection in Primary Lens Epithelial Cells

Hideharu OCHIAI1), Miyoko SAITO2), Takuya MARUO3) and Nobuyuki KANEMAKI3)
1) Research Institute of Biosciences, Azabu University, School of Veterinary Medicine 2) Laboratory of Veterinary Surgery 2, Azabu University, School of Veterinary Medicine 3) Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine
Abstract :

The full-length cDNA sequence of canine excitatory amino acid transporter (EAAT) 5 was determined in samples taken from the canine retina. The sequence was 2,467 bp long and was predicted to encode the 560 amino acid polypeptides. The deduced amino acid sequence of canine EAAT5 showed similarities of 91.8 and 92.7% to those of humans and rats, respectively. In canine, EAAT5 has a 49.4% identity with EAAT1, 43.7% with EAAT2, 46.4% with EAAT3, and 45.7% with EAAT4. RT-PCR analysis revealed EAAT5 expression in primary lens epithelial cell culture and the cerebellum, and Western blot analysis detected a single band of 60 kDa which confirmed EAAT5 protein expression in these cells. In addition, all subtypes of EAAT were detected in canine lens epithelial cells, indicating the pivotal role of EAATs in supplying glutamate, the precursor of antioxidant glutathione in the lens.

Keywords :
amino acid transporter, cDNA sequence, dog, retina

Date Deposited : 21 Jun 2011 14:32

Last Modified : 21 Jun 2011 14:32

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 4, - 2010

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