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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 1 > Hui Yao 1*, Yan Teng 2*, Qian Sun 1, Jing Xu 1, Ya-Tong Chen 1, Ning Hou 2, Xuan Cheng 2, Xiao Yang 2, Zhi-Nan Chen 1

Important Functional Roles of Basigin in Thymocyte Development and T cell Activation

Hui Yao 1*, Yan Teng 2*, Qian Sun 1, Jing Xu 1, Ya-Tong Chen 1, Ning Hou 2, Xuan Cheng 2, Xiao Yang 2, Zhi-Nan Chen 1
1. Cell Engineering Research Centre & Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, 169 West Changle Street, Xi'an 710032, China. 2. Genetic Laboratory of Development and Disease, Institute of Biotechnology, 20 Dongdajie, Fengtai District, Beijing 100071, China. * These authors contributed equally to this work.  Corresponding authors: Zhi-Nan Chen, Cell Engineering Research Centre & Department of Cell Biology, State Key Laboratory of Cancer Biology, Fourth Military Medical University, 169 West Changle Street, Xi'an 710032, China. Tel: +86-29-84773243; Fax: +86-29-83293906; E-mail: znchen@fmmu.edu.cn. Xiao Yang, Genetic Laboratory of Development and Disease, Institute of Biotechnology, 20 Dongdajie, Fengtai District, Beijing 100071, China. Tel: +86-10-63895937; Fax: +86-10-63895937; E-mail: yangx@nic.bmi.ac.cn.
Abstract :

Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we generated a conditional null allele by introducing two loxP sites flanking exons 2 and 7 of the basigin gene (Bsg). Bsgfl/fl mice were born at the expected Mendelian ratio and showed a similar growth rate compared with wildtype mice. After crossing these mice with Lck-Cre transgenic mice, basigin expression was specifically inactivated in T cells in the resulting Lck-Cre; Bsgfl/fl mice. Although the birth and growth rate of Lck-Cre; Bsgfl/fl mice were similar to control mice, thymus development was partially arrested in Lck-Cre; Bsgfl/fl mice, specifically at the CD4+ CD8+ double-positive (DP) and CD4 single-positive (CD4+ CD8- , CD4SP) stages. In addition, CD4+ T cell activation was enhanced upon Concanavalin A (Con A) or anti-CD3/anti-CD28 stimulation but not upon PMA/Ionomycin stimulation in the absence of basigin. Overall, this study provided the first in vivo evidence for the function of basigin in thymus development. Moreover, the successful generation of the conditional null basigin allele provides a useful tool for the study of distinct physiological or pathological functions of basigin in different tissues at different development stages.

Keywords :
Basigin; Cre-Loxp; gene targeting; thymocyte development; T cell activation

Date Deposited : 01 Feb 2016 10:34

Last Modified : 01 Feb 2016 10:34

Official URL: http://www.ijbs.com/v10i1

Volume 10, Number 1, - 2014 , ISSN 1449-2288

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