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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 2 > Jiang-chao Li1, Yongxia Yang2, Xiao-ming Zhou2, Si-mei Guo1, Ying Liu1, Cui-ling Qi1, Tian Lan1, Qian-qian Zhang1, Li-jing Wang1

Micrometastasis Expressing Insulin Arise in Lung and Spleen at Advanced Stage of Rip1-Tag2 Transgenic Mice

Jiang-chao Li1, Yongxia Yang2, Xiao-ming Zhou2, Si-mei Guo1, Ying Liu1, Cui-ling Qi1, Tian Lan1, Qian-qian Zhang1, Li-jing Wang1
1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China;  Corresponding author: Li-jing Wang, Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China. Tel: 86-20-39352126; E-mail: wanglijing@gdpu.edu.cn
Abstract :

Rip1-Tag2 mice is one overt pancreatic β-cell tumor model, which is widely used for studying pancreas tumor angiogenesis and tumor development. However, tumor metastasis in Rip1-Tag2 mice had rarely been reported, in this present study, we find some micrometastasis in lung and spleen of the Rip1-Tag2 mice at advanced stage, which is important for uncovering metastasis cell characteristics and exploring how to survive in cancer microenvironment. To study the micrometastasis of Rip1-Tag2 mice in advanced pancreatic cancer, we first observed the pathology process of β cell tumor in Rip1-Tag2 mice through HE staining, then we performed immunohistochemistry with insulin antibody, T-antigen antibodies and C-petide antibody on lung and spleen tissues sections from advanced stage, comparing with background wild-type C57BL/6 mice sections. The results indicated that micrometastasis expressing insulin was found in the Rip1-Tag2 mice lung, and spleen. Further evidences demonstrate pathology structure of lung and spleen are damaged. Interestingly and importantly, the expression of T antigen and insulin antibodies are all decreased in advanced stage of primary β cell tumor, which suggest that the at least partly micrometastasis is derived from the early stage or from advanced stage of β cell tumor then return to undifferentiated state like cancer stem cell. The findings contributed to the study of cancer metastasis and cancer stem cell.

Keywords :
micrometastasis, insulin, Rip1-Tag2 transgenic mice

Date Deposited : 02 Feb 2016 10:04

Last Modified : 02 Feb 2016 10:04

Official URL: http://www.ijbs.com/v10i2

Volume 10, Number 2, - 2014 , ISSN 1449-2288

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