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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 3 > Junzhe Jin, Guofu Lin, Hong Huang, Dong Xu, Hao Yu, Xu Ma, Lisi Zhu, Dongyan Ma, Honglei Jiang

Capsaicin Mediates Cell Cycle Arrest and Apoptosis in Human Colon Cancer Cells via Stabilizing and Activating p53

Junzhe Jin, Guofu Lin, Hong Huang, Dong Xu, Hao Yu, Xu Ma, Lisi Zhu, Dongyan Ma, Honglei Jiang
The Fourth Affiliated Hospital of China Medical University, Shenyang, China.  Corresponding author: Honglei Jiang Ph.D. M.D. Address: The No.4 Chongshan east road Huanggu district, Shenyang Liaoning, 110032. Tel: 024-62043517 Fax: 024-62255001 E-mail: grkjxz@sj-hospital.org
Abstract :

Capsaicin is the major pungent ingredient in red peppers which is world widely consumed. Except its potent pain relieving efficacy as reported, capsaicin also exerted its antitumor activity in several tumor models. Here, we reported that capsaicin had a profound anti-proliferative effect on human colon cancer cells via inducing cell cycle G0/G1 phase arrest and apoptosis, which was associated with an increase of p21, Bax and cleaved PARP. The underlying mechanism of capsaicin’s antitumor potency was mainly attributed to the stabilization and activation of p53. Capsaicin substantially prolonged the half-life of p53 and significantly elevated the transcriptional activity of p53. Through suppressing the interaction between p53 and MDM2, MDM2-mediated p53 ubiquitination was remarkably decreased after capsaicin treatment, which resulted in the stabilization and accumulation of p53. The results of p53-shRNA experiment further demonstrated that p53 knockdown severely impaired the sensitivity of tested cells to capsaicin, G0/G1 phase arrest and the apoptosis induced by capsaicin in p53-knockdown cells was also dramatically decreased, implicating the important role of p53 played in capsaicin’s antitumor activity. In summary, our data suggested that capsaicin, or a related analogue, may have a role in the management of human colon cancer.

Keywords :
colon cancer, capsaicin, p53, MDM2, ubiquitination.

Date Deposited : 03 Feb 2016 12:13

Last Modified : 03 Feb 2016 12:13

Official URL: http://www.ijbs.com/v10i3

Volume 10, Number 3, - 2014 , ISSN 1449-2288

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