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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 5 > Takashi SHIINA1), Keiko TANAKA1), Yoshihiko KATSUYAMA2), Kouji OTABE3), Kengo SAKAMOTO3), Masaaki KURATA3), Mamoru NOMURA3), Hisashi YAMANAKA3), Hiroshi NAKAGAWA3), Hidetoshi INOKO1) and Masao OTA4)

Mitochondrial DNA Diversity among Three Subpopulations of Cynomolgus Macaques (Macaca fascicularis) Originating from the Indochinese Region

Takashi SHIINA1), Keiko TANAKA1), Yoshihiko KATSUYAMA2), Kouji OTABE3), Kengo SAKAMOTO3), Masaaki KURATA3), Mamoru NOMURA3), Hisashi YAMANAKA3), Hiroshi NAKAGAWA3), Hidetoshi INOKO1) and Masao OTA4)
1) Department of Molecular Life Science, Tokai University School of Medicine 2) Department of Pharmacy, Shinshu University Hospital 3) Ina Research Inc. 4) Department of Legal Medicine, Shinshu University School of Medicine
Abstract :

The cynomolgus macaque (Macaca fascicularis) has emerged as an important experimental animal model for biomedical research in various domains, necessitating the more extensive characterization of the genetic backgrounds influencing the macaque’s response to drugs and sensitivity to experimental disease. The diversity of the variable mitochondrial DNA (mtDNA) D-loop region has been analyzed phylogenetically among geographically isolated populations or within subdivisions of the same regional population. However, the genetic differences among several substructures originating from a common population have not yet been investigated. By sequencing fragments of the mtDNA D-loop region from two subpopulations from the Indochinese region (Cambodian-Chinese and Vietnamese) along with two native Indonesian and Filipino populations, we identified 87 mtDNA D-loop haplotypes, of which 67 are new. The phylogenetic relationship suggests that the Indochinese haplotypes are intermingled in comparison to the distinct divergence of the Indonesian and Filipino lineages. The subpopulations were shown by estimation of evolutionary divergence and Wright’s F-statistic (Fst) to have little genetic differentiation. Altogether, the subpopulations may be used in biomedical research, even though a slight difference is observed in haplotype frequencies among them. Therefore, genetic diversity analyses will be necessary for the elucidation of genetic differences among the populations, as well as to obtain a better understanding of genetic diversity for biomedical research. This will involve the selection of macaques and the monitoring of genetic heterogeneity among and within breeding facilities.

Keywords :
cynomolgus macaques, genetic diversity, mtDNA, phylogenetic relationship, population structure

Date Deposited : 21 Jun 2011 15:23

Last Modified : 21 Jun 2011 15:23

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 5, - 2010

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