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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 5 > FuYing LIU1), JingLi SHANG1), ShuXia SONG1), Long ZHENG1), KaiXia ZHANG1), HongYu YOU1), HuanLing ZHANG1) and JunXia WANG1)

Augmented Induction of Antigen-Specific Cytotoxic T Cell Responses against Canine Hepatitis by Co-Immunization with pVAX1-CpG-Loop and Adjuvants in BALB/c Mice

FuYing LIU1), JingLi SHANG1), ShuXia SONG1), Long ZHENG1), KaiXia ZHANG1), HongYu YOU1), HuanLing ZHANG1) and JunXia WANG1)
1) Hebei Key Lab of Laboratory Animal, Department of Molecular Biology, Hebei Medical Universit
Abstract :

The objective of this study was to obtain better antigen specific cytotoxic T cell responses in vivo. We examined the augmented induction of antigen-specific cytotoxic T cell responses to co-administration of oligonucleotides (CpG-ODN), dimethyl dioctadecyl ammonium bromide (DDA), and Lipofectamine™ 2000 with a DNA vaccine (pVAX1-CpG-Loop) and boosting with pVAX1-CpG-Loop in BALB/c mice. The results show that Loop protein-specific T cell proliferation, cytotoxic T cell activity, and the production of CD8+ T cells and IFN-γ were enhanced after co-immunization of mice with adjuvants and pVAX1-CpG-Loop. We demonstrated that significant T cell-mediated immune responses were induced in the mice with the help of DDA, CpG-ODN and Lipofectamine™ 2000.

Keywords :
adjuvants, BALB/c mice, cytotoxic T cell responses, plasmid pVAX1-CpG-Loop

Date Deposited : 21 Jun 2011 15:25

Last Modified : 21 Jun 2011 15:25

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 5, - 2010

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