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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 7 > Hao Ding, Song Chen, Wen-Qi Song, You-Shui Gao, Jun-Jie Guan, Yang Wang, Yuan Sun and Chang-Qing Zhang

Dimethyloxaloylglycine Improves Angiogenic Activity of Bone Marrow Stromal Cells in the Tissue-Engineered Bone

Hao Ding, Song Chen, Wen-Qi Song, You-Shui Gao, Jun-Jie Guan, Yang Wang, Yuan Sun and Chang-Qing Zhang
Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China These authors provided equal contribution to this work.  Corresponding authors: Chang-Qing Zhang (E-mail: zhangcq@sjtu.edu.cn), Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital, 600 Yishan Road, Shanghai, China; Yuan Sun (E-mail: david1979982@163.com), Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital, 600 Yishan Road, Shanghai, China
Abstract :

One of the big challenges in tissue engineering for treating large bone defects is to promote the angiogenesis of the tissue-engineered bone. Hypoxia inducible factor-1α (HIF-1α) plays an important role in angiogenesis-osteogenesis coupling during bone regeneration, and can activate a broad array of angiogenic factors. Dimethyloxaloylglycine (DMOG) can activate HIF-1α expression in cells at normal oxygen tension. In this study, we explored the effect of DMOG on the angiogenic activity of bone mesenchymal stem cells (BMSCs) in the tissue-engineered bone. The effect of different concentrations of DMOG on HIF-1a expression in BMSCs was detected with western blotting, and the mRNA expression and secretion of related angiogenic factors in DMOG-treated BMSCs were respectively analyzed using qRT-PCR and enzyme linked immunosorbent assay. The tissue-engineered bone constructed with β-tricalcium phosphate (β-TCP) and DMOG-treated BMSCs were implanted into the critical-sized calvarial defects to test the effectiveness of DMOG in improving the angiogenic activity of BMSCs in the tissue-engineered bone. The results showed DMOG significantly enhanced the mRNA expression and secretion of related angiogenic factors in BMSCs by activating the expression of HIF-1α. More newly formed blood vessels were observed in the group treated with β-TCP and DMOG-treated BMSCs than in other groups. And there were also more bone regeneration in the group treated with β-TCP and DMOG-treated BMSCs. Therefore, we believed DMOG could enhance the angiogenic activity of BMSCs by activating the expression of HIF-1α, thereby improve the angiogenesis of the tissue-engineered bone and its bone healing capacity.

Keywords :
angiogenesis, bone marrow stromal cells, bone substitutes, dimethyloxaloylglycine, hypoxia inducible factor-1α

Date Deposited : 12 Feb 2016 10:16

Last Modified : 12 Feb 2016 10:16

Official URL: http://www.ijbs.com/v10i7

Volume 10, Number 7, - 2014 , ISSN 1449-2288

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