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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 8 > Alicia Vázquez1, Juan de Dios Ruiz-Rosado1, Luis I. Terrazas1, Imelda Juárez1, Lorena Gomez-Garcia2, Elsa Calleja1, Griselda Camacho1, Ana Chávez1, Miriam Romero1, Tonathiu Rodriguez1, Bertha Espinoza3 and Miriam Rodriguez-Sosa1

Mouse Macrophage Galactose-type Lectin (mMGL) is Critical for Host Resistance against Trypanosoma cruzi Infection

Alicia Vázquez1, Juan de Dios Ruiz-Rosado1, Luis I. Terrazas1, Imelda Juárez1, Lorena Gomez-Garcia2, Elsa Calleja1, Griselda Camacho1, Ana Chávez1, Miriam Romero1, Tonathiu Rodriguez1, Bertha Espinoza3 and Miriam Rodriguez-Sosa1
1. Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México (UNAM), C. P. 54090, Estado de México, México. 2. Department of Immunology, Instituto Nacional de Cardiología “Ignacio Chávez,” México, D.F. 14080 México. 3. Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México. México, D.F. 04510 México.  Corresponding author: Miriam Rodríguez-Sosa, Unidad de Biomedicina, FES-Iztacala, UNAM. Av. de los Barrios # 1, Los Reyes Iztacala, 54090 Tlalnepantla, Edo. de México. Mexico. Phone: (52-55) 5623-1333 ext. 39789. Fax: (52-55) 5623-1225. Email: rodriguezm@campus.iztacala.unam.mx.
Abstract :

The C-type lectin receptor mMGL is expressed exclusively by myeloid antigen presenting cells (APC) such as dendritic cells (DC) and macrophages (Mφ), and it mediates binding to glycoproteins carrying terminal galactose and α- or β-N-acetylgalactosamine (Gal/GalNAc) residues. Trypanosoma cruzi (T. cruzi) expresses large amounts of mucin (TcMUC)-like glycoproteins. Here, we show by lectin-blot that galactose moieties are also expressed on the surface of T. cruzi. Male mMGL knockout (-/-) and wild-type (WT) C57BL/6 mice were infected intraperitoneally with 104 T. cruzi trypomastigotes (Queretaro strain). Following T. cruzi infection, mMGL-/- mice developed higher parasitemia and higher mortality rates compared with WT mice. Although hearts from T. cruzi-infected WT mice presented few amastigote nests, mMGL-/- mice displayed higher numbers of amastigote nests. Compared with WT, Mφ from mMGL-/- mice had low production of nitric oxide (NO), interleukin (IL)-12 and tumor necrosis factor (TNF)-α in response to soluble T. cruzi antigens (TcAg). Interestingly, upon in vitro T. cruzi infection, mMGL-/- Mφ expressed lower levels of MHC-II and TLR-4 and harbored higher numbers of parasites, even when mMGL-/- Mφ were previously primed with IFN-γ or LPS/IFN-γ. These data suggest that mMGL plays an important role during T. cruzi infection, is required for optimal Mφ activation, and may synergize with TLR-4-induced pathways to produce TNF-α, IL-1β and NO during the early phase of infection.

Keywords :
mMGL, Trypanosoma cruzi, Proinflammatory cytokines, C-Type lectin receptor, Macrophages receptors

Date Deposited : 12 Feb 2016 10:58

Last Modified : 12 Feb 2016 10:58

Official URL: http://www.ijbs.com/v10i8

Volume 10, Number 8, - 2014 , ISSN 1449-2288

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