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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2014 > Volume 10 Number 9 > Ying Wang 2*, Yang Zhang2*, Huimin Wang2, Junxiao Wang1, Yiyuan Zhang2, Yingzhe Wang2, Zhenwei Pan2, Shanshun Luo1

Aberrantly Up-regulated miR-20a in Pre-eclampsic Placenta Compromised the Proliferative and Invasive Behaviors of Trophoblast Cells by Targeting Forkhead Box Protein A1

Ying Wang 2*, Yang Zhang2*, Huimin Wang2, Junxiao Wang1, Yiyuan Zhang2, Yingzhe Wang2, Zhenwei Pan2, Shanshun Luo1
1. Department of Gerontology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P. R. China; 2. Department of Pharmacology (Key Laboratory of Cardiovascular Medicine Research, Ministry of Education; State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin, Heilongjiang 150081, P. R. China. * Equal contributions to the work.  Corresponding author: Dr. Luo Shanshun luoss63@126.com Department of Gerontology, The First Affiliated Hospital of Harbin Medical University, or Dr. Pan Zhenwei panzw@ems.hrbmu.edu.cn. Department of Pharmacology, Harbin Medical University, Baojian Road 157, Harbin, Heilongjiang 150081, P. R. China. Fax: 86 451 86675769 or 86667511 Tel: 86 451 86671354.
Abstract :

Preeclampsia is a serious complication in pregnancy. Dysregulation of trophoblast cell proliferation and invasion is a major pathological alteration observed in preeclampsia. Recently, microRNAs were shown to participate in the pathogenesis of preeclampsia. In this study we explored the effect of miR-20a on the proliferation and invasion of trophoblast cells and the underlying mechanism. We verified the distribution of miR-20a in human placenta by in situ hybridization. Real time PCR data showed that the level of miR-20a increased by 2.6 folds in human preeclampsia than normal tissues. We then cultured trophoblast-like JEG-3 cells and evaluated the effect of miR-20a on JEG-3 cell proliferation, migration and invasion. Overexpression of miR-20a significantly inhibited the proliferation, migration and invasion of cultured JEG-3 cells, which were abolished by co-transfection of AMO-20a. Transfection of miR-20a also inhibited JEG-3 cell xenograft tumor growth in nude mice. Luciferase assay technique was used to identify the direct regulation of miR-20a on Forkhead Box Protein A1(FOXA1). Transfection of miR-20a markedly reduced the luciferase activity of the chimeric plasmid containing the 3’UTR of FOXA1, indicating FOXA1 is the target of miR-20a. Furthermore, transfection of miR-20a inhibited both protein and mRNA expression of FOXA1 in JEG-3 cells. In summary, the upregulated miR-20a in human preeclampsia tissue can inhibit the proliferative and invasive activities of trophoblast cells by repressing the expression of FOXA1

Keywords :
FOXA1; invasion; miR-20a; migration; proliferation.

Date Deposited : 13 Feb 2016 10:18

Last Modified : 13 Feb 2016 10:18

Official URL: http://www.ijbs.com/v10i9

Volume 10, Number 9, - 2014 , ISSN 1449-2288

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