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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 5 > Hiroyuki ISHITOBI1), Ken MATSUMOTO1), Takuya AZAMI1), Fumiko ITOH2), Susumu ITOH2)3), Satoru TAKAHASHI1) and Masatsugu EMA1)

Flk1-GFP BAC Tg Mice: An Animal Model for the Study of Blood Vessel Development

Hiroyuki ISHITOBI1), Ken MATSUMOTO1), Takuya AZAMI1), Fumiko ITOH2), Susumu ITOH2)3), Satoru TAKAHASHI1) and Masatsugu EMA1)
1) Department of Anatomy and Embryology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba 2) Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba 3) High Technology Research Center, Showa Pharmaceutical University Laboratory of Biochemistry, Showa Pharmaceutical University
Abstract :

The mouse Flk1 (also called Kdr or Vegf-r2) gene encodes a receptor for VEGF-A. Flk1 is expressed in endothelial cells of the developing embryo. Recent studies have shown that Flk1 is expressed by multi-potent mesodermal progenitors, which give rise to various hematopoietic and cardiovascular cell lineages during development, and in differentiating ES cells, which may be used for cell transplantation therapy to treat cardiovascular diseases. Given its developmental and clinical importance in cardiovascular tissues, an animal model of Flk1 activity would be very useful. Here, we report the generation of Flk1-GFP BAC transgenic mice for monitoring Flk1 gene expression during development. We show that GFP expression in these mice serves as a surrogate marker for developing endothelial cells. Immunohistochemical analysis showed that the regions of expression of GFP and endogenous FLK1 largely overlap. Uniform GFP expression was observed in most endothelial cells at 8.5 dpc and thereafter. Flk1-GFP BAC transgenic mice should be useful for the study of both vascular development and pathological angiogenesis.

Keywords :
angiogenesis, cardiogenesis, Flk1, vasculogenesis, VEGF-A

Date Deposited : 21 Jun 2011 15:32

Last Modified : 21 Jun 2011 15:32

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 5, - 2010

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