International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 1 > Xin Yi, Yanbo Zhang, Peidan Wang, Jiangwei Qi, Meiying Hu, Guohua Zhong
Ligands Binding and Molecular Simulation: the Potential Investigation of a Biosensor Based on an Insect Odorant Binding Protein
Xin Yi, Yanbo Zhang, Peidan Wang, Jiangwei Qi, Meiying Hu, Guohua Zhong
Laboratory of Insect Toxicology, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, People’s Republic of China. Corresponding author: Prof GuoHua Zhong. Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, PR China, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. E-mail: email@example.com. Tel: +86-20-85280308; Fax: +86-20-85280292.
Based on mimicking biological olfaction, biosensors have been applied for the detection of various ligands in complex environment, which could represent one of the most promising research fields. In this study, the basic characters of one insect odorant binding protein (OBP) as a biosensor were explored. To explore the molecular recognition process, the tertiary structure of the protein was modeled and the protein-ligand interactions with 1,536,550 chemicals were investigated by the molecular docking. The availability of large amount of recombinant SlitOBP1 overcame the difficulty to obtain biological sensing material. After obtained the purified recombinant protein, the result of fluorescence binding assays proved the candidate protein has good affinities with the majority of the tested chemicals. With the aid of simulation docking, the key conserved amino acids within the binding site were identified and then mutated to alanine. After mutation, the protein-ligand binding characteristics were recorded, and the competitive binding assays were carried out to provide experimental verification. The detailed information on its structure and affinities investigated in this study could allow the design of specific mutants with desired characteristics, which provides a solid base for tailoring OBP for biosensor and provides a role model for screening the other elements in olfactory system for different applications.
Odorant binding protein, Biosensors, Molecular simulation, Fluorescence binding, Site-directed mutagenesis
Date Deposited : 15 Feb 2016 11:56
Official URL: http://www.ijbs.com/v11i1
Last Modified : 15 Feb 2016 11:56
Volume 11, Number 1, - 2015 , ISSN 1449-2288
Full Text Original