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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 5 > Osamu HASHIMOTO1), Tomohiro YONEZAWA2), Yuka SUGIYAMA1), Mitsumori KAWAMINAMI2) and Yoshihisa HASEGAWA1)

Molecular Cloning and Expression of Canine Prolactin Gene

Osamu HASHIMOTO1), Tomohiro YONEZAWA2), Yuka SUGIYAMA1), Mitsumori KAWAMINAMI2) and Yoshihisa HASEGAWA1)
1) Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine 2) Laboratory of Veterinary Physiology, Kitasato University, School of Veterinary Medicine
Abstract :

We isolated canine prolactin cDNA from a dog pituitary cDNA library. The 930-bp nucleotide sequence covered the entire open reading frame encoding the putative 229 amino acids. It was located in chromosome 35, and had five exons. The amino acid sequence was highly homologous to the feline and porcine sequences. To generate recombinant canine prolactin, plasmids for full-length canine prolactin were constructed and transfected into the mammalian HEK293 cell line. The recombinant prolactin was secreted into the medium as an N-linked glycosylated (31 kDa) or non-glycosylated (27 kDa) protein. Western blotting revealed both of these bands were canine pituitary protein extracts.

Keywords :
dog, pituitary, recombinant protein

Date Deposited : 21 Jun 2011 15:38

Last Modified : 21 Jun 2011 15:38

Official URL: http://www.jstage.jst.go.jp/browse/expanim/60/1/_contents

Volume 59, Number 5, - 2010

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