UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 3 > Nazmul Haque1,2, Noor Hayaty Abu Kasim1,2, , Mohammad Tariqur Rahman3

Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells

Nazmul Haque1,2, Noor Hayaty Abu Kasim1,2, , Mohammad Tariqur Rahman3
1. Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia. 2. Regenerative Dentistry Research Group, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia. 3. Department of Biotechnology, Faculty of Science, International Islamic University Malaysia, Kuantan, Malaysia.  Corresponding authors: Mohammad Tariqur Rahman, Ph.D., Associate Professor, Department of Biotechnology, Faculty of Science, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan 25200, Pahang, Malaysia. Phone: +601399494741 (Mobile), +6095705042 (Office); Fax: +6095726781; Email: tarique@iium.edu.my | m.tariqur.rahman@gmail.com or Noor Hayaty Abu Kasim, Professor, Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia, Tel. No. (Office): +6-03-79674806, Fax No.: +6-03-79674533, E-mail: nhayaty@um.edu.my
Abstract :

Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits.

Keywords :
Mesenchymal stem cell, hyper-immunogenicity, chemokine receptors, xenogenic, autologous, allogeneic

Date Deposited : 22 Feb 2016 09:50

Last Modified : 22 Feb 2016 09:50

Official URL: http://www.ijbs.com/v11i3

Volume 11, Number 3, - 2015 , ISSN 1449-2288

Download:
Full Text Original
Abstract : pdf doc