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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 1 > Ranjna S. Cheema, Amrit K. Bansal*, G.S. Bilaspuri , V. K. Gandotra

Correlation between the proteins and protein profile(s) of different regions of epididymis and their contents in goat buck

Ranjna S. Cheema, Amrit K. Bansal*, G.S. Bilaspuri , V. K. Gandotra
Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University,Ludhiana 141004, India
Abstract :

Total proteins and their profile(s) in three regions of epididymis, its fluid and sperm membrane
extract (SME) were analysed with an objective to determine the changes in spermatozoa during
epididymal maturation in goat buck. The protein content from caput to cauda increased nonsignificantly
(P>0.05) while significantly (P<0.05) in tissue homogenate and spermatozoa. SDSPAGE
analysis indicated the removal of >205, 205 and 95 kDa; 40 kDa proteins only in the caput
and corpus spermatozoa, respectively. The proteins with molecular weight of 10, 18, 25, 35 and 20
kDa, detected only in cauda and corpus spermatozoa, respectively, seem to be associated in the
maturation process of spermatozoa during epididymal transit. SDS-Page analysis of tissue and fluid
indicated that the >205, 205, 195, 200, 45, 25, 18, 15 and 12 kDa proteins are structural as well as
secretory proteins because of their presence both in the epididymal tissue and fluid. The nature of
100, 97, 75 and 70 kDa was found to be purely structural because of their presence in the tissue only,
whereas 90, 35, 6.5 and 3.0 kDa proteins, mainly of secretory nature, due to their detection only in
the fluid. Therefore, the presence of 35, 25, 18 kDa proteins both in the epididymal fluid and cauda
sperm indicate that these proteins are associated with goat buck sperm maturation and fertility.
Therefore, it can be concluded that the proteins of 35, 25, and 18 kDa, associated with goat sperm
maturation are similar to the mentioned molecular markers in other species and can be worked out
as fertility markers for goat buck semen.

Keywords :
bucks / detergents / epididymis / proteins / spermatozoa

Date Deposited : 21 Jun 2011 15:59

Last Modified : 21 Jun 2011 15:59

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Volume 29, Number 1, - 2011

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