UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 6 > Lihua Sun, Chao Xu, Guoqing Chen, Min Yu, Songwei Yang, Yuan Qiu, Ke Peng, Wensheng Wang, Weidong Xiao, Hua Yang

A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway

Lihua Sun, Chao Xu, Guoqing Chen, Min Yu, Songwei Yang, Yuan Qiu, Ke Peng, Wensheng Wang, Weidong Xiao, Hua Yang
Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, China.  Corresponding authors: Hua Yang, M.D., Ph.D. or Weidong Xiao, M.D., Ph.D.. Department of General Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China, Phone: (0086)23-687-55705 (Office), E-mail: huayang@tmmu.edu.cn or xiaoweidong@tmmu.edu.cn
Abstract :

OS-9 is a lectin required for efficient ubquitination of glycosylated substrates of endoplasmic reticulum-associated degradation (ERAD). OS-9 has previously been implicated in ER-to-Golgi transport and transcription factor turnover. However, we know very little about other functions of OS-9 under endoplasmic reticulum stress. Here, we used gene knockdown and overexpression approaches to study the protective effect of OS-9 on intestinal barrier function of intestinal epithelial cell Caco-2 monolayer. We found that OS-9 attenuated intestinal epithelial barrier dysfunction under hypoxia through up-regulating occludin and claudin-1 protein expression. Furthermore, we showed that the up-regulation of occludin and claudin-1 induced by OS-9 was mediated by p38 and ERK1/2 phosphorylation and did not involve HIF-1α. In summary, our results demonstrate that OS-9 up-regulates occludin and claudin-1 by activating the MAP kinase (MAPK) pathway, and thus protects the epithelial barrier function of Caco-2 monolayer under hypoxia condition.

Keywords :
OS-9; intestinal epithelial barrier; p38; tight junction

Date Deposited : 23 Feb 2016 13:18

Last Modified : 23 Feb 2016 13:18

Official URL: http://www.ijbs.com/v11i6

Volume 11, Number 6, - 2015 , ISSN 1449-2288

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal