UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 6 > Wen-Hua Li1,2, Li Zhou1, Zhi Li1, Yang Wang1, Jian-Tao Shi1, Yan-Jing Yang1,2 and Jian-Fang Gui1

Zebrafish Lbh-like Is Required for Otx2-mediated Photoreceptor Differentiation

Wen-Hua Li1,2, Li Zhou1, Zhi Li1, Yang Wang1, Jian-Tao Shi1, Yan-Jing Yang1,2 and Jian-Fang Gui1
1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 2. Graduate University of the Chinese Academy of Sciences, Wuhan 430072, China  Corresponding authors: Jian-Fang Gui and Li Zhou. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China; Tel.: +86-27-68780707; Fax: +86-27-68780123. E-mail: jfgui@ihb.ac.cn and zhouli@ihb.ac.cn
Abstract :

The homeobox transcription factor orthodenticle homolog 2 (otx2) is supposed as an organizer that orchestrates a transcription factor network during photoreceptor development. However, its regulation in the process remains unclear. In this study, we have identified a zebrafish limb bud and heart-like gene (lbh-like), which is expressed initially at 30 hours post fertilization (hpf) in the developing brain and eyes. Lbh-like knockdown by morpholinos specifically inhibits expression of multiple photoreceptor-specific genes, such as opsins, gnat1, gnat2 and irbp. Interestingly, otx2 expression in the morphants is not significantly reduced until 32 hpf when lbh-like begins to express, but its expression level in 72 hpf morphants is higher than that in wild type embryos. Co-injection of otx2 and its downstream target neuroD mRNAs can rescue the faults in eyes of Lbh-like morphants. Combined with the results of promoter-reporter assay, we suggest that lbh-like is a new regulator of photoreceptor differentiation directly through affecting otx2 expression in zebrafish. Furthermore, knockdown of lbh-like increases the activity of Notch pathway and perturbs the balance among proliferation, differentiation and survival of photoreceptor precursors.

Keywords :
Lbh-like, retina development, photoreceptor differentiation, otx2, Notch

Date Deposited : 23 Feb 2016 13:21

Last Modified : 23 Feb 2016 13:21

Official URL: http://www.ijbs.com/v11i6

Volume 11, Number 6, - 2015 , ISSN 1449-2288

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal