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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 1 > J.X. Liu1, 2, G. Ren1, H. Chen1**, F. Li1, X.Y. Lan1, M.J. Li1, J.Q. Wang3

Five novel SNPs of the bovine LHX4 gene and their association with growth traits in native Chinese cattle breeds

J.X. Liu1, 2, G. Ren1, H. Chen1**, F. Li1, X.Y. Lan1, M.J. Li1, J.Q. Wang3
1 College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China 2 College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, 832002, China 3 Research Center of Cattle Engineering Technology in Henan; Zhengzhou, Henan, 450003, P.R. China
Abstract :

LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some
mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth
and development in animal. The objective of this study is revealing to these polymorphisms of in
LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms
in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene
showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T,
g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M
(G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four
cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies
significantly differed among the four analyzed populations (***P<0.001). The association analysis
showed that individuals with genotype MM had greater body weight than those with genotype MN
(*P<0.05) at eighteen months of age in Nanyang cattle.

Keywords :
growth traits / SNPs / LHX4 gene / PCR-SSCP

Date Deposited : 21 Jun 2011 16:03

Last Modified : 21 Jun 2011 16:03

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Volume 29, Number 1, - 2011

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