UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 7 > Hongxue Shi 1, Yi Cheng 1, Jingjing Ye 1, Pingtao Cai 1, Jinjing Zhang 1, Rui Li 1, Ying Yang 1, Zhouguang Wang 1, Hongyu Zhang 1, Cai Lin 2, Xianghong Lu 3, Liping Jiang 1, Aiping Hu 1, Xinbo Zhu 1, Qiqiang Zeng 2, Xiaobing Fu 4, Xiaokun Li 1, Jian Xiao 1

bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways

Hongxue Shi 1, Yi Cheng 1, Jingjing Ye 1, Pingtao Cai 1, Jinjing Zhang 1, Rui Li 1, Ying Yang 1, Zhouguang Wang 1, Hongyu Zhang 1, Cai Lin 2, Xianghong Lu 3, Liping Jiang 1, Aiping Hu 1, Xinbo Zhu 1, Qiqiang Zeng 2, Xiaobing Fu 4, Xiaokun Li 1, Jian Xiao 1
1. School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035 China 2. The First Affiliate Hospital, Wenzhou Medical University, Wenzhou, 325035, China 3. Translation Medicine Research Center, Lishui People’s Hospital, Wenzhou Medical University, Lishui, 323000, China 4. Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, PR China  Corresponding authors: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035 China. Tel.: +86-577-85773087; Fax.: +86-577-85773087; E-mail address: xfxj2000@126.com (J. Xiao), xiaokunli@163.net (X. Li)
Abstract :

Fibroblasts play a pivotal role in the process of cutaneous wound repair, whereas their migratory ability under diabetic conditions is markedly reduced. In this study, we investigated the effect of basic fibroblast growth factor (bFGF) on human dermal fibroblast migration in a high-glucose environment. bFGF significantly increased dermal fibroblast migration by increasing the percentage of fibroblasts with a high polarity index and reorganizing F-actin. A significant increase in intracellular reactive oxygen species (ROS) was observed in dermal fibroblasts under diabetic conditions following bFGF treatment. The blockage of bFGF-induced ROS production by either the ROS scavenger N-acetyl-L-cysteine (NAC) or the NADPH oxidase inhibitor diphenylene iodonium chloride (DPI) almost completely neutralized the increased migration rate of dermal fibroblasts promoted by bFGF. Akt, Rac1 and JNK were rapidly activated by bFGF in dermal fibroblasts, and bFGF-induced ROS production and promoted dermal fibroblast migration were significantly attenuated when suppressed respectively. In addition, bFGF-induced increase in ROS production was indispensable for the activation of focal adhesion kinase (FAK) and paxillin. Therefore, our data suggested that bFGF promotes the migration of human dermal fibroblasts under diabetic conditions through increased ROS production via the PI3K/Akt-Rac1-JNK pathways.

Keywords :
bFGF, human dermal fibroblast

Date Deposited : 25 Feb 2016 11:50

Last Modified : 25 Feb 2016 11:50

Official URL: http://www.ijbs.com/v11i7

Volume 11, Number 7, - 2015 , ISSN 1449-2288

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal