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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 1 > Jianbin Li1,2*, Hongmei Wang2, Yi Zhang1, Minghai Hou2, Jifeng Zhong2*, Yuan Zhang1

Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle

Jianbin Li1,2*, Hongmei Wang2, Yi Zhang1, Minghai Hou2, Jifeng Zhong2*, Yuan Zhang1
1 College of Animal Science and Technology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100094 China 2 Dairy Cattle Research Centre, Shandong Academy of Agricultural Science, 159-1 Gongye North Road, Jinan, 250100 China
Abstract :

Bovine leukocyte adhesion deficiency (BLAD) and citrullinemia are two autosomal recessive genetic
diseases in Holstein cattle and both result in death of homozygous animals. Through the polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology and DNA
sequencing, one citrullinemia and three BLAD carriers were found in a population of 615 Chinese
Holstein cattle, including 436 cows and 179 bulls. Citrullinemia and BLAD carrier frequency was
0.16% and 0.49%, respectively, in tested Chinese Holstein cattle. In this study, the first citrullinemia
carrier occurring in Chinese Holstein cattle is described, although the world frequency of this
disorder is very low.

Keywords :
BLAD / citrullinemia / Chinese Holstein cattle / PCR-RFLP

Date Deposited : 21 Jun 2011 16:04

Last Modified : 21 Jun 2011 16:04

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Volume 29, Number 1, - 2011

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