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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 8 > Xiangjun Song, Xiaomin Zhao, Yong Huang, Hailing Xiang, Wenlong Zhang, Dewen Tong

Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7

Xiangjun Song, Xiaomin Zhao, Yong Huang, Hailing Xiang, Wenlong Zhang, Dewen Tong
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China These authors contributed equally to this work. Corresponding author: Prof. Dewen Tong. Telephone: +86-29-87091622; Fax: +86-29-87091032; E-mail: dwtong@nwsuaf.edu.cn
Abstract :

Transmissible gastroenteritis virus (TGEV) is a member of Coronaviridae family. TGEV infection has emerged as a major cause of severe gastroenteritis and leads to alterations of many cellular processes. Meanwhile, the pathogenic mechanism of TGEV is still unclear. microRNAs (miRNAs) are a novel class of small non-coding RNAs which are involved in the regulation of numerous biological processes such as viral infection and cell apoptosis. Accumulating data show that miRNAs are involved in the process of coronavirus infection such as replication of severe acute respiratory syndrome coronavirus (SARS-CoV). However, the link between miRNAs and TGEV infection is unknown. In this study, we performed microRNA microarray assay and predicted targets of altered miRNAs. The results showed TGEV infection caused the change of miRNAs profile. Then we selected miR-4331 for further analysis and subsequently identified cell division cycle-associated protein 7 (CDCA7) as the target of miR-4331. Moreover, miR-4331 showed the ability to inhibit transcription of TGEV gene 7 (a non-structure gene) via directly targeting CDCA7. In conclusion, differentially expressed miR-4331 that is caused by TGEV infection can suppress transcription of TGEV gene 7 via targeting cellular CDCA7. Our key finding is that TGEV selectively manipulates the expression of some cellular miRNAs to regulate its subgenomic transcription.

Keywords :
TGEV; microRNAs (miRNAs); TGEV; CDCA7; Gene 7; viral transcription

Date Deposited : 01 Mar 2016 10:47

Last Modified : 01 Mar 2016 10:47

Official URL: http://www.ijbs.com/v11i8

Volume 11, Number 8, - 2015 , ISSN 1449-2288

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