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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 8 > Hak-Bong Kim1, Su-Hoon Lee1, Jee-Hyun Um2, Mi-Ju Kim1, Suh-Kyung Hyun1, Eun-Ji Gong1, Won Keun Oh3, Chi-Dug Kang1, Sun-Hee Kim1

Sensitization of Chemo-Resistant Human Chronic Myeloid Leukemia Stem-Like Cells to Hsp90 Inhibitor by SIRT1 Inhibition

Hak-Bong Kim1, Su-Hoon Lee1, Jee-Hyun Um2, Mi-Ju Kim1, Suh-Kyung Hyun1, Eun-Ji Gong1, Won Keun Oh3, Chi-Dug Kang1, Sun-Hee Kim1
1. Department of Biochemistry, Pusan National University School of Medicine, Yangsan 626-870, Korea 2. Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, Korea 3. College of Pharmacy, Seoul National University, Seoul 151-818, Korea These authors contributed equally to this study as corresponding authors. Corresponding authors: Sun-Hee Kim and Chi-Dug Kang, Department of Biochemistry, Pusan National University School of Medicine, Yangsan 626-870, Republic of Korea. Tel.: +82-51-510-8081. Fax: +82-51-510-8086, E-mail address: ksh7738@pusan.ac.kr (Sun-Hee Kim), kcdshbw@pusan.ac.kr (Chi-Dug Kang).
Abstract :

Development of effective therapeutic strategies to eliminate cancer stem-like cells (CSCs), which play a major role in drug resistance and disease recurrence, is critical to improve cancer treatment outcomes. The current investigation was undertaken to examine the effectiveness of the combination treatment of Hsp90 inhibitor and SIRT1 inhibitor in inhibiting the growth of chemo-resistant stem-like cells isolated from human chronic myeloid leukemia K562 cells. Inhibition of SIRT1 by use of SIRT1 siRNA or SIRT1 inhibitors (amurensin G and EX527) effectively potentiated sensitivity of Hsp90 inhibitors (17-AAG and AUY922) in CD44high K562 stem-like cells expressing high levels of CSC-related molecules including Oct4, CD34, β-catenin, c-Myc, mutant p53 (mut p53), BCRP and P-glycoprotein (P-gp) as well as CD44. SIRT1 depletion caused significant down-regulation of heat shock factor 1 (HSF1)/heat shock proteins (Hsps) as well as these CSC-related molecules, which led to the sensitization of CD44high K562 cells to Hsp90 inhibitor by SIRT1 inhibitor. Moreover, 17-AAG-mediated activation of HSF1/Hsps and P-gp-mediated efflux, major causes of Hsp90 inhibitor resistance, was suppressed by SIRT1 inhibitor in K562-CD44high cells. Our data suggest that combined treatment with Hsp90 inhibitor and SIRT1 inhibitor could be an effective therapeutic approach to target CSCs that are resistant to current therapies.

Keywords :
Hsp90 inhibitor, K562, CD44, SIRT1 inhibitor, multidrug resistance, cancer stem-like cells

Date Deposited : 01 Mar 2016 10:49

Last Modified : 01 Mar 2016 10:49

Official URL: http://www.ijbs.com/v11i8

Volume 11, Number 8, - 2015 , ISSN 1449-2288

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