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.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 8 > Yuanyuan Zhang, Minhui Sun, Yuwei Han, Kefeng Zhai, Youmei Tang, Xiaoying Qin, Zhengyu Cao, Boyang Yu, Junping Kou

The saponin DT-13 Attenuates Tumor Necrosis Factor-α-induced Vascular Inflammation Associated with Src/NF-кB/MAPK Pathway Modulation

Yuanyuan Zhang, Minhui Sun, Yuwei Han, Kefeng Zhai, Youmei Tang, Xiaoying Qin, Zhengyu Cao, Boyang Yu, Junping Kou
Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China Co-first authors (Y.Z.,& M.S.) Corresponding authors: Dr. Boyang Yu & Dr. Junping Kou, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China, Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China. Tel&Fax: +86- 25- 86185158; E-mail: boyangyu59@163.com& junpingkou@cpu.edu.cn
Abstract :

This study aimed to explore the effect of DT-13 (25(R,S)-ruscogenin- 1-O- [β-d-glucopyranosyl- (1→2)][β-d-xylopyranosyl-(1→3)]-β -d- fucopyranoside) on tumor necrosis factor (TNF)-α-induced vascular inflammation and the potential molecular mechanisms. In vitro, DT-13 suppressed TNF-α-induced adhesion and migration of human umbilical vein endothelial cells (HUVECs) by inhibiting the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). DT-13 markedly suppressed NF-кB p65 phosphorylation, and when NF-кB p65 was over-expressed, the inhibitory effect of DT-13 on adhesion molecular decreased. DT-13 also suppressed TNF-α induced luciferase activities of ICAM-1 and VCAM-1 promoter containing NF-κB binding sites. Furthermore, DT-13 markedly suppressed p38 phosphorylation and Src degradation induced by TNF-α, whereas had no significant effect on ERK and JNK activation. In vivo, DT-13 at 4 mg/kg prevented vascular inflammation and the expression of adhesion molecules induced by TNF-α in mice. These findings suggest that DT-13 abrogates vascular inflammation by down-regulating adhesion molecules associated with modulating the NF-кB, p38MAPK, Src signaling pathways, and NF-κB binding site is at least one of the targets of DT-13. This study provides novel information regarding the mechanism by which DT-13 exerts its effects on vascular inflammation, which is important for the onset and progression of various diseases.

Keywords :
DT-13; Endothelial inflammation; NF-кB; Src; MAPKs

Date Deposited : 01 Mar 2016 10:56

Last Modified : 01 Mar 2016 10:56

Official URL: http://www.ijbs.com/v11i8

Volume 11, Number 8, - 2015 , ISSN 1449-2288

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