UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 9 > Zeng-Fu Shang1,2, Wei Tan2,3, Xiao-Dan Liu2, Lan Yu4, Bing Li2, Ming Li1, Man Song1,2, Yu Wang2, Bei-Bei Xiao1, Cai-Gao Zhong3, Hua Guan2, Ping-Kun Zhou1,2

DNA-PKcs Negatively Regulates Cyclin B1 Protein Stability through Facilitating Its Ubiquitination Mediated by Cdh1-APC/C Pathway

Zeng-Fu Shang1,2, Wei Tan2,3, Xiao-Dan Liu2, Lan Yu4, Bing Li2, Ming Li1, Man Song1,2, Yu Wang2, Bei-Bei Xiao1, Cai-Gao Zhong3, Hua Guan2, Ping-Kun Zhou1,2
1. School of Radiation Medicine and Protection, Medical College of Soochow University; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, Jiangsu 215123, P. R. China 2. Department of Radiation Toxicology and Oncology, Beijing Key Laboratory for Radiobiology (BKLRB), Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China 3. School of Public Heath, Central South University, Changsha, Hunan Province, Changsha, Hunan 410078, P. R. China 4. Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA These authors contributed equally to this work Corresponding authors: Ping-Kun Zhou, E-mail: zhoupk@bmi.ac.cn; Hua Guan, Email: ghlsh@163.com
Abstract :

The catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is a critical component of the non-homologous end-joining pathway of DNA double-stranded break repair. DNA-PKcs has also been shown recently functioning in mitotic regulation. Here, we report that DNA-PKcs negatively regulates the stability of Cyclin B1 protein through facilitating its ubiquitination mediated by Cdh1 / E 3 ubiquitin ligase APC/C pathway. Loss of DNA-PKcs causes abnormal accumulation of Cyclin B1 protein. Cyclin B1 degradation is delayed in DNA-PKcs-deficient cells as result of attenuated ubiquitination. The impact of DNA-PKcs on Cyclin B1 stability relies on its kinase activity. Our study further reveals that DNA-PKcs interacts with APC/C core component APC2 and its co-activator Cdh1. The destruction of Cdh1 is accelerated in the absence of DNA-PKcs. Moreover, overexpression of exogenous Cdh1 can reverse the increase of Cyclin B1 protein in DNA-PKcs-deficient cells. Thus, DNA-PKcs, in addition to its direct role in DNA damage repair, functions in mitotic progression at least partially through regulating the stability of Cyclin B1 protein

Keywords :
Cyclin B1, DNA-PKcs

Date Deposited : 02 Mar 2016 11:41

Last Modified : 02 Mar 2016 11:41

Official URL: http://www.ijbs.com/v11i9

Volume 11, Number 9, - 2015 , ISSN 1449-2288

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal