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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 9 > Yu Li1, Ke Wang1, Hui Xie1, Dong-Wei Wang1, Chun-Ling Xu1, Xin Huang1, Wen-Jia Wu1, Dan-Lei Li2

Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis

Yu Li1, Ke Wang1, Hui Xie1, Dong-Wei Wang1, Chun-Ling Xu1, Xin Huang1, Wen-Jia Wu1, Dan-Lei Li2
1. Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China; 2. College of Forestry, Northeast Forestry University, Haerbin 150040, China These authors contributed equally to this work Corresponding author: Prof. Xie Hui, Tele: 86-20-38297286, Fax: 86-20-38297286, E-mail: xiehui@scau.edu.cn
Abstract :

Radopholus similis is an important plant parasitic nematode which severely harms many crops. Cathepsin B is present in a wide variety of organisms, and plays an important role in many parasites. Understanding cathepsin B of R. similis would allow us to find new targets and approaches for its control. In this study, we found that Rs-cb-1 mRNA was expressed in esophageal glands, intestines and gonads of females, testes of males, juveniles and eggs in R. similis. Rs-cb-1 expression was the highest in females, followed by juveniles and eggs, and was the lowest in males. The maximal enzyme activity of Rs-CB-1 was detected at pH 6.0 and 40 °C. Silencing of Rs-cb-1 using in vitro RNAi (Soaking with dsRNA in vitro) not only significantly inhibited the development and hatching of R. similis, but also greatly reduced its pathogenicity. Using in planta RNAi, we confirmed that Rs-cb-1 expression in nematodes were significantly suppressed and the resistance to R. similis was significantly improved in T2 generation transgenic tobacco plants expressing Rs-cb-1 dsRNA. The genetic effects of in planta RNAi-induced gene silencing could be maintained in the absence of dsRNA for at least two generations before being lost, which was not the case for the effects induced by in vitro RNAi. Overall, our results first indicate that Rs-cb-1 plays key roles in the development, hatching and pathogenesis of R. similis, and that in planta RNAi is an effective tool in studying gene function and genetic engineering of plant resistance to migratory plant parasitic nematodes

Keywords :
Radopholus similis, Cathepsin B, development, pathogenesis, RNAi, transgenic tobacco

Date Deposited : 04 Mar 2016 12:51

Last Modified : 04 Mar 2016 12:51

Official URL: http://www.ijbs.com/v11i9

Volume 11, Number 9, - 2015 , ISSN 1449-2288

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