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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 10 > Xiangying Kong1, Yue Yang1, Wenbin Wu1, Hongye Wan1, Xiaomin Li1, Michun Zhong1, Xiaohui Su1, Shiwei Jia2, Na Lin 1

Triterpenoid Saponin W3 from Anemone flaccida Suppresses Osteoclast Differentiation through Inhibiting Activation of MAPKs and NF-κB Pathways

Xiangying Kong1, Yue Yang1, Wenbin Wu1, Hongye Wan1, Xiaomin Li1, Michun Zhong1, Xiaohui Su1, Shiwei Jia2, Na Lin 1
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China. 2. Guangzhou Kanghe Pharmaceutical Limited Company, 511440 Guangzhou, China Corresponding author: Na Lin. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing 100700, China. Tel:+861064014411-2869; Fax: +861064013996. E-mail: linna888@163.com
Abstract :

Excessive bone resorption by osteoclasts within inflamed joints is the most specific hallmark of rheumatoid arthritis. A. flaccida has long been used for the treatment of arthritis in folk medicine of China; however, the active ingredients responsible for the anti-arthritis effects of A. flaccida are still elusive. In this study, W3, a saponin isolated from the extract of A. flaccida was identified as the major active ingredient by using an osteoclast formation model induced by receptor activator of nuclear factor kappa-B ligand (RANKL). W3 dose-dependently suppressed the actin ring formation and lacunar resorption. Mechanistic investigation revealed that W3 inhibited the RANKL-induced TRAF6 expression, decreased phosphorylation of mitogen-activated protein kinases (MAPKs) and IκB-α, and suppressed NF-κB p65 DNA binding activity. Furthermore, W3 almost abrogated the expression of c-Fos and nuclear factor of activated T cells (NFATc1). Therefore, our results suggest that W3 is a potential agent for treating lytic bone diseases although further evaluation in vivo and in clinical trials is needed.

Keywords :
Anemone flaccida; RANKL; Osteoclasts; Bone resorption

Date Deposited : 08 Mar 2016 10:40

Last Modified : 08 Mar 2016 10:40

Official URL: http://www.ijbs.com/v11i10

Volume 11, Number 10, - 2015 , ISSN 1449-2288

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