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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 11 > Liang Xia, Caixing Sun, Qinglin Li, Fang Feng, Enqi Qiao, Limin Jiang, Bin Wu, Minghua Ge

CELF1 is Up-Regulated in Glioma and Promotes Glioma Cell Proliferation by Suppression of CDKN1B

Liang Xia, Caixing Sun, Qinglin Li, Fang Feng, Enqi Qiao, Limin Jiang, Bin Wu, Minghua Ge
Zhejiang Cancer Hospital, Hangzhou 310022, Zhejiang Province, P.R.C Liang Xia, Caixing Sun and Qinglin Li contributed equally to this work. Corresponding authors: Bin Wu M.D. Email: wubinbindoc@163.com; Minghua Ge M.D. Email: gemingh@163.com; Limin Jiang M.D. Email:jlm129@163.com. Address: Zhejiang Cancer Hospital, 38 Guangji Road, Hangzhou 310022, Zhejiang Province, P.R.C., Phone: +86-571-88122222
Abstract :

BACKGROUND: As a member of the CELF family, CELF1 (CUG-binding protein 1, CUGBP1) is involved in cardiac and embryonic development, skeletal muscle differentiation and mammary epithelial cell proliferation. CELF1 is also observed in many kinds of cancer and may play a great role in tumorigenesis and deterioration. However, the expression and mechanism of its function in human glioma remain unclear. METHODS: We examined CELF1 expression in 62 glioma patients by immunohistochemistry and Western blot. The association between the expression of CELF1 protein and clinicopathological characteristics was analysed using SPSS 17.0. Survival analyses were performed using the Kaplan–Meier method. Small-interfering RNA was utilised to specifically knockdown CELF1 mRNA in U87 and U251 cells. Cell proliferation, cell cycle and cell apoptosis were tested by Cell Counting Kit-8 and flow cytometry. The expression of cell cycle-related gene CDKN1B was investigated by Western blot. The interactions between CELF1 and CDKN1B were detected with immune co-precipitation. Subcutaneous tumour models were used to study the effect of CELF1 on the growth of glioma cells in vivo. RESULTS: Our results showed that CELF1 protein was frequently up-regulated in human glioma tissues. The expression level of this protein was positively correlated with glioma World Health Organisation grade and inversely correlated with patient survival (P < 0.05). Knockdown of CELF1 inhibited the glioma cell cycle process and proliferation potential, possibly by down-regulating its target, CDKN1B protein. CONCLUSIONS: Results indicated that CELF1 may be a novel independent prognostic predictor of survival for glioma patients. It may promote glioma cell proliferation and cell cycle process during glioma carcinogenesis.

Keywords :
CELF1, Glioma, Prognosis, Proliferation

Date Deposited : 08 Mar 2016 11:08

Last Modified : 08 Mar 2016 11:08

Official URL: http://www.ijbs.com/v11i11

Volume 11, Number 11, - 2015 , ISSN 1449-2288

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