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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 2 > Shamim Akhter**, Bushra Allah Rakha, Syed Murtaza Hasan Andrabi, Muhammad Sajjad Ansari

Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa

Shamim Akhter**, Bushra Allah Rakha, Syed Murtaza Hasan Andrabi, Muhammad Sajjad Ansari
Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan
Abstract :

The present study was designed to compare egg yolks from three avian species in extender for
cryopreservation of Sahiwal bull epididymal spermatozoa. The study was conducted on cauda
epididymal spermatozoa from ten slaughtered Sahiwal bulls. Semen retrieved from epididymal
cauda of each testes pair was pooled and diluted in tris-citric acid-glycerol extender containing either
pigeon, guinea fowl or chicken egg yolk semi colon leading to a final spermatozoal concentration of
30×106 ml-1. Diluted semen was transferred into 0.5 ml straws, cooled to 4°C and equilibrated for 4
h. The straws were kept on liquid nitrogen vapours for 10 min and then plunged into liquid nitrogen
for storage. Motility, plasma membrane integrity and spermatozoa morphology (acrosome ridge;
head, mid piece and tail) of each diluted semen sample were assessed at 0, 2 and 4 h post-thawing.
All indicators applied and acrosomal integrity were the highest (P<0.05) after freezing in extender
containing pigeon egg yolk compared to guinea fowl and chicken egg yolk 0, 2 and at 4 h postthaw.
Moreover, spermatozoa tail abnormality percentages were also significantly lower (P<0.05)
in extender containing pigeon egg yolk compared to guinea fowl and chicken egg yolk at 0, 2 and 4
post-thaw h. In conclusion, pigeon egg yolk used in semen extender improved the post-thaw quality
of Sahiwal bull epididymal spermatozoa.

Keywords :
bulls / cattle / cryopreservation / egg yolk / epididymal spermatozoa / extender / Sahiwal breed / semen

Date Deposited : 21 Jun 2011 16:09

Last Modified : 21 Jun 2011 16:11

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 29, Number 2, - 2011

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