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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2015 > Volume 11 Number 12 > Zhaoliang Liu1, 2, Mario G. Oyola1, Suoling Zhou1, Xian Chen1, Lan Liao1, Jean Ching-Yi Tien1, Shailaja K. Mani1, Jianming Xu1, 3

Knockout of the Histone Demethylase Kdm3b Decreases Spermatogenesis and Impairs Male Sexual Behaviors

Zhaoliang Liu1, 2, Mario G. Oyola1, Suoling Zhou1, Xian Chen1, Lan Liao1, Jean Ching-Yi Tien1, Shailaja K. Mani1, Jianming Xu1, 3
1. Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, USA. 2. Institute of Cancer Research, Harbin Medical University, Harbin, China. 3. Institute for Cancer Medicine and College of Basic Medical Sciences, Sichuan Medical University, Luzhou, Sichuan, China Corresponding author: Jianming Xu, PhD, Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Phone: 713-798-6199; Fax: 713-798-3017; E-mail: jxu@bcm.edu
Abstract :

Kdm3b is a JmjC domain-containing histone H3 (H3) demethylase and its physiological functions are largely unknown. In this study, we found that Kdm3b protein is highly expressed in multiple cell types in the mouse testes, including Leydig cells, Sertoli cells, spermatogonia and spermatocytes at different differentiation stages. We also observed Kdm3b protein in the epithelial cells of the caput epididymis, prostate and seminal vesicle. Breeding tests revealed that the number of pups produced by the breeding pairs with Kdm3b knockout (Kdm3bKO) males and wild type (WT) females was reduced 68% because of the decreased number of litters when compared with the breeding pairs with WT males and females. Further analysis demonstrated that Kdm3bKO male mice produced 44% fewer number of mature sperm in their cauda epididymides, displaying significantly reduced sperm motility. No significant differences in the circulating concentration of testosterone and the expression levels of androgen receptor and its representative target genes in the testis were observed. However, the circulating levels of 17β-estradiol, a modulator of sperm maturation and male sexual behaviors, was markedly reduced in Kdm3bKO male mice. Strikingly, abrogation of Kdm3b in male mice significantly increased the latencies to mount, intromit and ejaculate and decreased the number of mounts and intromissions, largely due to their loss of interest in female odors. These findings indicate that Kdm3b is required for normal spermatogenesis and sexual behaviors in male mice.

Keywords :
Kdm3b, knockout mice, testis, reproductive function, sexual behavior

Date Deposited : 08 Mar 2016 13:39

Last Modified : 08 Mar 2016 13:39

Official URL: http://www.ijbs.com/v11i12

Volume 11, Number 12, - 2015 , ISSN 1449-2288

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