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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 2 > El؟bieta Wirth-Dziêcio³owska1,3, Ma³gorzata Zimowska2, Marta.Gajewska3, Monika ¯muda1, Stanis³aw J. Rosochacki4, **

Differential growth of skeletal muscle in mice selected divergently over 108 generations for low and high body weight

El؟bieta Wirth-Dziêcio³owska1,3, Ma³gorzata Zimowska2, Marta.Gajewska3, Monika ¯muda1, Stanis³aw J. Rosochacki4, **
1 Warsaw Agricultural University, Department of Genetics and Animal Breeding, Ciszewskiego 8, 02-786 Warszawa, Poland 2 Warsaw University, Faculty of Biology, Institute of Zoology, Department of Cytology, Miecznikowa 1, 02-096 Warszawa, Poland 3 The Maria Sk³odowska-Curie Memorial Cancer Centre and Institute of Oncology in Warsaw Roentgena 5, 02-781 Warszawa, Poland 4 Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland and Bia³ystok Technical University, Chair of Sanitary Biology and Biotechnology, Wiejska 45e, 15-351 Bia³ystok, Poland
Abstract :

Experiments were performed on 180 mice from two lines dubbed light (L) and heavy (C), selected
divergently for body weight over 108 generations. The main hypothesis was that the changes
occurring in body weight and muscle weight as a result of directed divergent selection could be
associated with changes in the transcription of some miogenic genes and/or with the level of proteins
regulating myogenesis and with the composition of muscle fibres.
Hind limb muscle masses from females and males of the two lines were weighed at 1 and 3 weeks and
3 month of age. Morphological analysis for histological cross-sections of the gastroenemius muscle
was carried out in 3-week and 3-month-old mice. The percentage comparison of nuclei in muscle
fibres were analysed, too. Levels of MYOD1, MYF-5 and myogenin at the same time points were
determined using Western blotting. Microsatellite markers for MyoD1, Myf-5 and MYOG were used
to compare allele frequencies of analysed genes in both lines. There were differences in muscleweight between the sexes at age of 3 months. Muscles of the hind limbs were heavier in males than
in females by 23.7% in line C, and by 14% in line L. Significant differences in muscle mass were
accompanied by changes in muscle fibre size. The number of large-diameter muscle fibres increased
with animals’ age, and in females fibres of diameters of 60-80 ىm accounted for 38% of the total in
line C, as compared with 94% preponderance of smaller (20-60 ىm) fibres in the muscle of line L
females. The numbers of nuclei were clearly greater in line C than in line L individuals, as well as in
3-month-old animals as compared with those at 3 weeks of age.
Selection have brought about change, not only in myogenesis, but also in the frequency of alleles
of microsatellite markers MyoD1, Myf-5 and at the myogenin locus, thus suggesting that molecular
differences between the lines have arisen. Differences in the levels of MYOD1, MYF5 and MYOG
are evident between both sexes and the selected lines of mice.

Keywords :
mice / body weight / divergent selection / myogenesis / fibre size / MyoD

Date Deposited : 21 Jun 2011 16:10

Last Modified : 21 Jun 2011 16:10

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Volume 29, Number 2, - 2011

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