International Journal Of Bilogical Sciences
.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 2 > Chong Wang, Chang-Ming Liu, Li-Liang Wei, Li-Ying Shi, Zhi-Fen Pan, Lian-Gen Mao, Xiao-Chen Wan, Ze-Peng Ping, Ting-Ting Jiang, Zhong-Liang Chen, Zhong-Jie Li, Ji-Cheng Li
A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing
Chong Wang, Chang-Ming Liu, Li-Liang Wei, Li-Ying Shi, Zhi-Fen Pan, Lian-Gen Mao, Xiao-Chen Wan, Ze-Peng Ping, Ting-Ting Jiang, Zhong-Liang Chen, Zhong-Jie Li, Ji-Cheng Li
1. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China 2. Department of Respiratory Medicine, The Sixth Hospital of Shaoxing, Shaoxing 312000, P.R. China 3. Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, P.R. China 4. Department of Tuberculosis, The First Hospital of Jiaxing, Jiaxing 314001, P.R. China Corresponding author: Dr. Ji-Cheng Li, Zhejiang University School of Medicine, 866 Yuhangtang Road, Hangzhou 310058, P.R. China. Tel: +86 571-88208088; Fax: +86 571-88208088. Email: firstname.lastname@example.org.
The epidemic of pulmonary tuberculosis (TB), especially multidrug-resistance tuberculosis (MDR-TB) presented a major challenge for TB treatment today. We performed iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) and Solexa sequencing among MDR-TB patients, drug-sensitive tuberculosis (DS-TB) patients, and healthy controls. A total of 50 differentially expressed proteins and 43 differentially expressed miRNAs (fold change >1.50 or <0.60, P<0.05) were identified in the MDR-TB patients compared to both DS-TB patients and healthy controls. We found that 22.00% of differentially expressed proteins and 32.56% of differentially expressed miRNAs were related, and could construct a network mainly in complement and coagulation cascades. Significant differences in CD44 antigen (CD44), coagulation factor XI (F11), kininogen-1 (KNG1), miR-4433b-5p, miR-424-5p, and miR-199b-5p were found among MDR-TB patients, DS-TB patients and healthy controls (P<0.05) by enzyme-linked immunosorbent assay (ELISA) and SYBR green qRT-PCR validation. A strong negative correlation, consistent with the target gene prediction, was found between miR-199b-5p and KNG1 (r=-0.232, P=0.017). Moreover, we established the MDR-TB diagnostic model based on five biomarkers (CD44, KNG1, miR-4433b-5p, miR-424-5p, and miR-199b-5p). Our study proposes potential biomarkers for MDR-TB diagnosis, and also provides a new experimental basis to understand the pathogenesis of MDR-TB.
multidrug-resistance tuberculosis; proteomic; transcriptomic; serum; biomarkers
Date Deposited : 15 Mar 2016 14:17
Official URL: http://www.ijbs.com/v12i2
Last Modified : 15 Mar 2016 14:17
Volume 12, Number 2, - 2016 , ISSN 1449-2288
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