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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2016 > Volume 12 Number 4 > Mengtao Ma, Miao He, Qian Jiang, Yuanyuan Yan, Shu Guan, Jing Zhang, Zhaojin Yu, Qiuchen Chen, Mingli Sun, Weifan Yao, Haishan Zhao, Feng Jin, Minjie Wei

MiR-487a Promotes TGF-β1-induced EMT, the Migration and Invasion of Breast Cancer Cells by Directly Targeting MAGI2

Mengtao Ma, Miao He, Qian Jiang, Yuanyuan Yan, Shu Guan, Jing Zhang, Zhaojin Yu, Qiuchen Chen, Mingli Sun, Weifan Yao, Haishan Zhao, Feng Jin, Minjie Wei
1. Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning Province, China; 2. Department of Surgical Oncology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China. These authors contributed equally to this work. Corresponding author: Minjie Wei; Department of Pharmacology, School of Pharmacy, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang 110122, Liaoning Province, P.R. China. Tel: +86/24/23256666-5318; Fax: +86/24/23255471. E-mail address: weiminjiecmu@163.com
Abstract :

Tumor metastasis is a complex and multistep process and its exact molecular mechanisms remain unclear. We attempted to find novel microRNAs (miRNAs) contributing to the migration and invasion of breast cancer cells. In this study, we found that the expression of miR-487a was higher in MDA-MB-231breast cancer cells with high metastasis ability than MCF-7 breast cancer cells with low metastasis ability and the treatment with transforming growth factor β1 (TGF-β1) significantly increased the expression of miR-487a in MCF-7 and MDA-MB-231 breast cancer cells. Subsequently, we found that the transfection of miR-487a inhibitor significantly decreased the expression of vimentin, a mesenchymal marker, while increased the expression of E-cadherin, an epithelial marker, in both MCF-7 cells and MDA-MB-231 cells. Also, the inactivation of miR-487a inhibited the migration and invasion of breast cancer cells. Furthermore, our findings demonstrated that miR-487a directly targeted the MAGI2 involved in the stability of PTEN. The down-regulation of miR-487a increased the expression of p-PTEN and PTEN, and reduced the expression of p-AKT in both cell lines. In addition, the results showed that NF-kappaB (p65) significantly increased the miR-487a promoter activity and expression, and TGF-β1 induced the increased miR-487a promoter activity via p65 in MCF-7 cells and MDA-MB-231 cells. Moreover, we further confirmed the expression of miR-487a was positively correlated with the lymph nodes metastasis and negatively correlated with the expression of MAGI2 in human breast cancer tissues. Overall, our results suggested that miR-487a could promote the TGF-β1-induced EMT, the migration and invasion of breast cancer cells by directly targeting MAGI2.

Keywords :
miR-487a; MAGI2; EMT; TGF-β1; p65; Breast cancer.

Date Deposited : 16 Mar 2016 11:44

Last Modified : 16 Mar 2016 11:44

Official URL: http://www.ijbs.com/v12i4

Volume 12, Number 4, - 2016 , ISSN 1449-2288

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