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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 2 > Jiajie Sun1, Chunlei Zhang1, Xingtang Fang1, Chuzhao Lei2, Xianyong Lan2, Hong Chen1

The polymorphism of bovine FIT gene and its associations with cattle (Bos taurus) growth traits

Jiajie Sun1, Chunlei Zhang1, Xingtang Fang1, Chuzhao Lei2, Xianyong Lan2, Hong Chen1
1 Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China 2 College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China
Abstract :

Fat-inducing transcript (FIT) are endoplasmic reticulum-resident membrane proteins that induce lipid
droplet accumulation. It plays a crucial role in the fundamental process of storing fat. In this study,
applying the PCR-SSCP and DNA sequencing methods, polymorphism of the FIT gene were detected.
A total of 708 individuals from four Chinese cattle breeds were examined. The results showed that only
P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.199G > T resulting in
L124L) and a missense mutation (NM_001103095: m.434G > T resulting in V176L). The associations
between polymorphic loci and selected growth traits of indigenous Nangyang cattle were analysed,
and significant associations were found in body weight at the age of 12 months and mean daily live
weight gain. The body weight at month 12 of life and mean daily live weight gain of individuals with
genotype AA were by 3.75% and 4.88% higher than of those with genotype AB, respectively. Hence, it
was suggested for the first time, that genotype AA could be regarded as molecular marker for superior
body weight and daily live weight gain in Chinese Nanyang cattle.

Keywords :
association analysis / FIT gene / gene polymorphism / SSCP / SNP

Date Deposited : 21 Jun 2011 16:16

Last Modified : 21 Jun 2011 16:16

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Volume 29, Number 2, - 2011

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