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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 3 > Caode Jiang1a**, Shun Li1a, Ping Shi1a, Ranran Dong1, Jiawei Tian1, Changyan Deng2

Characterization and imprinting analysis of COPG2 and MEST genes in pigs

Caode Jiang1a**, Shun Li1a, Ping Shi1a, Ranran Dong1, Jiawei Tian1, Changyan Deng2
1 School of Life Science, Southwest University, Chongqing 400715, China 2 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, China
Abstract :

MEST and COPG2 in human MEST imprinted cluster are two of good candidate genes responsible
for primordial growth retardation including Silver–Russell syndrome. In order to increase
understanding of these genes in pigs, their cDNAs are characterized in this report. By real-time
quantitative RT-PCR and polymorphism-based method, tissue and allelic expression of both genes
were determined using F1 reciprocal Landrace × Rongchang pig crossbreds. The transcription
levels of MEST differed between tissues and decreased as development proceeded. The gene was
imprinted and paternally expressed in heart, stomach, skeletal muscle, kidney, lung, bladder,
tongue and fat, while biallelic expression was detected in liver, small intestine and spleen of onemonth-
old pigs. The porcine COPG2 was differentially expressed between neonatal tissues and
showed biallelic expression in postnatal tissues. Furthermore, the transcript of COPG2 in bladder
and small intestine increased with age. It is concluded that tissue expression of porcine MEST is
similar to, while COPG2 differs from other mammalian homologues. In addition, porcine MEST
has development-specific imprinting, but imprinting of COPG2 in mammals is controversial.

Keywords :
COPG2 / gene / genomic imprinting / MEST / tissue expression / pig

Date Deposited : 21 Jun 2011 16:17

Last Modified : 21 Jun 2011 16:26

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Volume 29, Number 3, - 2011

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