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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 3 > Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter

Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen

Muhammad Sajjad Ansari, Bushra Allah Rakha, Shamim Akhter
Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University of Ravalpindi, Ravalpindi-46300, Pakistan
Abstract :

The study was conducted to evaluate the effect of addition of L-cysteine to tris-citric acid (TCA)
extender on the post-thaw quality of Sahiwal bull semen. For this purpose, two consecutive
ejaculates were collected from three Sahiwal bulls using artificial vagina at weekly intervals for a
period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile
spermatozoa ml-1) in TCA extender having L-cysteine either 0.0 (control) or 0.5, 1.0 or 2.0 mM.
Diluted semen was cooled to 4°C for 2 h, equilibrated for 4 h at 4°C, filled in straws at 4°C, kept in
liquid nitrogen vapours for 10 min and then stored in the liquid nitrogen. Thawing was performed
after 24 h of storage, at 37°C for 30 s. and the sperm motility, viability, plasma membrane and
acrosomal integrity were assessed. Higher (P<0.05) sperm motility, viability, plasma membrane and
acrosomal integrity were observed using extenders containing 1.0 or 2.0 mM compared to those
containing 0.5 or 0.0 mM L-cysteine. It is concluded that addition of L-cysteine (to reach 1.0-2.0
mM) in TCA extender improves the post-thaw quality of Sahiwal bull semen.

Keywords :
bull semen / cryopreservation / cysteine / Sahiwal / semen quality

Date Deposited : 21 Jun 2011 16:18

Last Modified : 21 Jun 2011 16:26

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 29, Number 3, - 2011

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