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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 3 > Mariusz Pierzcha³a1*, Chandra Shekhar Pareek2, Pawe³ Lisowski1, Pawe³ Urbaٌski1, Dorota Goluch1, Urszula Czarnik3, Marian Kamyczek4, Marian Rَ؟ycki5, Ross Gordon Cooper6, Jolanta Kuryl1

Expression profile of MYF5 and MYF6 genes in skeletal muscles of young growing gilts of five breeds at different ages, based on the most stable reference genes

Mariusz Pierzcha³a1*, Chandra Shekhar Pareek2, Pawe³ Lisowski1, Pawe³ Urbaٌski1, Dorota Goluch1, Urszula Czarnik3, Marian Kamyczek4, Marian Rَ؟ycki5, Ross Gordon Cooper6, Jolanta Kuryl1
1 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 2 Laboratory of Functional Genomics, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 11, 87-100 Torun, Poland 3 Department of Animal Genetics, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland 4 Experiment Station Pawlowice, National Research Institute of Animal Production, Krakَw, 64-122 Paw³owice, Poland 5 Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Cracov, 32-083 Balice, Poland 6 Physiologist, Eurohouse, Dog Kennel Lane, Walsall WS1 2BU, England, UK
Abstract :

The expression profile was evaluated of MYF5 and MYF6 genes in skeletal muscles of young growing
Polish Large White (PLW), Polish Landrace (PL), Pietrain (PIE), Duroc (DUR) and Pulawska
(PUL) gilts at different ages. Normalization of MYF5 and MYF6 was performed on reliable porcine
reference genes (PRGs), where expression stabilities of nine of them (ACTB, B2M, GAPDH, SDHA,
HPRTI, RPL13A, YWHAZ, TBP, TOP2B) were evaluated by RT-qPCR method and NormFindersoftware. Results revealed HPRTI, TBP and TOP2B as highly stable and PRGs. The age-dependent
and breed-specific skeletal muscle expression comparisons revealed highly significant (P<0.01)
differences in MYF6 expression levels of all skeletal muscles among investigated breeds. MYF6
gene expression in PIE and DUR were higher compared to PLW, PL and PUL gilts. Contrarily,
paired-wise comparison of MYF5 gene expression showed only significant difference between DUR
and PUL for semimembranosus, and PL and PLW, DUR and PL, PIE and PL, DUR and PUL and
PIE and PUL for gluteus medius muscle. There was no significant relationship identified between
gilt ages and the level of expression of MYF5 and MYF6 genes. However, their highest expression
was identified in longissimus dorsi followed by gluteus medius and semimembranosus muscles. It is
concluded that normalization of gene expression has to be done on more than one PRG to reduce the
errors in transcription level estimates. Moreover, significantly different breed-specific expression of
porcine MYF5 and MYF6 allowed the authors to prioritize these genes as potential candidate genes
for trait-associated study.

Keywords :
breed / development stage /gen expression / muscle / pig / RT-qPCR

Date Deposited : 21 Jun 2011 16:20

Last Modified : 21 Jun 2011 16:27

Official URL: http://www.ighz.edu.pl/?p0=5&p1=34

Volume 29, Number 3, - 2011

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