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.: Home > Animal Science Papers and Reports > 2015 > Volume 33 Number 2 > Jernej Ogorevc 1 , Sonja Prpar Mihevc 1 , Jakob Hedegaard 2 , Dušan Benčina 1 , Peter Dovč 1

Transcriptomic response of goat mammary epithelial cells to Mycoplasma agalactiae challenge – a preliminary study

Jernej Ogorevc 1 , Sonja Prpar Mihevc 1 , Jakob Hedegaard 2 , Dušan Benčina 1 , Peter Dovč 1
1 Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia 2 Department of Molecular Biology and Genetics, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark (Present address: Department of Molecular Medicine, Aarhus University Hospital, Skejby, Palle Jull-Jensens Boulevard 99, DK-8200 Aarhus N, Denmark)
Abstract :
Mycoplasma agalactiae
(
Ma
) is one of the main aetiological agents of intramammary infections in
small ruminants, causing contagious agalactia. To better understand the underlying disease patterns
a primary goat mammary epithelial cell (pgMEC) culture was established from the mammary
tissue and challenged with
Ma
. High-throughput mRNA sequencing was performed to reveal
differentially expressed genes (DEG) at different time-points (3 h, 12 h, and 24 h) post infection (PI).
The pathway enrichment analysis of the DEG showed that infection significantly affected pathways
associated with immune response, steroid metabolism, fatty acid metabolism, apoptosis signalling,
transcription regulation, and cell cycle regulation. Based on the results we suggest that mammary
epithelial cells
in vivo
contribute to the immune system by the induced expression of cytokines
and other chemotactic agents, activation of the complement system and apoptosis pathways, and
expression of genes coding for antimicrobial molecules and peptides. In our study we attempted to
interpret the detected transcriptomic changes in a biological context and infer mammary infection
156
resistance candidate genes, interesting for further validation. Additionally, the results represent
comprehensive goat mammary transcriptome information and demonstrate the applicability of the
comparative genomics approach for annotation of goat data, using transcriptome information of a
closely related species (Bos taurus) as a reference.
Keywords :
cell culture, high-throughput RNA sequencing, mammary gland, mastitis , Mycoplasma agalactiae

Date Deposited : 24 Mar 2016 10:40

Last Modified : 24 Mar 2016 10:40

Official URL: http://www.ighz.edu.pl/files/objects/7556/66/str_155-164.pdf

Volume 33, Number 2, March 2015

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