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.: Home > Animal Science Papers and Reports > 2015 > Volume 33 Number 3 > Juan Li 1, Jannik Ejnar Jakobsen 2 , Qiang Xiong 1 , Hang Fu 1 , Henrik Callesen 3

Developmental competence of porcine chimeric embryos produced by aggregation

Juan Li 1, Jannik Ejnar Jakobsen 2 , Qiang Xiong 1 , Hang Fu 1 , Henrik Callesen 3
1 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing Weigang 1, Jiangsu Province, China 2 Department of Biomedicine, Aarhus University, DK-8000 Aarhus C, Denmark 3 Department of Animal Science, Aarhus University, DK-8830 Tjele, Denmark
Abstract :
The purpose of our study was to compare the developmental competence and blastomere allocation
of porcine chimeric embryos formed by micro-well aggregation. Chimeras were created by
aggregating either two blastomeres originating from 2-cell embryos or two whole embryos, where
embryos were produced either by parthenogenetic activation (PA) or handmade cloning (HMC).
Results showed that the developmental competence of chimeric embryos, evaluated based on their
blastocyst rate and total cell number per blastocyst, was increased when two whole 2-cell stage
embryos (PA or HMC) were aggregated. In comparison, when two blastomeres were aggregated,
the developmental competence of the chimeric embryos decreased if the blastomeres were either
from PA or from HMC embryos, but not if they were from different sources, i.e. one PA and one
HMC blastomere. To evaluate the cell contribution in embryo formation, aggregation was made
with HMC embryos cloned using EGFP transgenic cells; the cell contribution in the formation of the
inner cell mass or trophectoderm was random in chimeric blastocysts. Finally, two blastomeres from
2-cell stage embryos were fused to construct tetraploid embryos, and when diploid and tetraploid
embryos were used for aggregation, the cells of the ICM and its close vicinity were mostly formed
by blastomeres from the diploid embryo.
Keywords :
chimera / co-culture / porcine / somatic cell nuclear transfer /traploidy / wow

Date Deposited : 24 Mar 2016 10:53

Last Modified : 24 Mar 2016 10:53

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Volume 33, Number 3, May 2015

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