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.: Home > Animal Science Papers and Reports > 2015 > Volume 33 Number 3 > Katarzyna Piórkowska 1, Joanna Nowak 2 , Katarzyna Połtowicz 2 , Katarzyna Ropka-Molik 1 , Tomasz Szmatoła 1

Effect of newly found polymorphisms in the promoter region of the CAPN1 gene on transcript abundance in broiler chicken breast muscle

Katarzyna Piórkowska 1, Joanna Nowak 2 , Katarzyna Połtowicz 2 , Katarzyna Ropka-Molik 1 , Tomasz Szmatoła 1
1 Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland 2 Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
Abstract :
The calpain family includes proteolytic enzymes, which have a high capacity to degrade cytoskeletal
and muscle fibre proteins. Thus they play an important role in the fusion of myoblasts and in cell
proliferation and growth. The CAPN1 gene has been selected as a ‘candidate gene’ for meat quality
in many domestic animals, including chickens. Consequently, the aim of our study was to identify
new polymorphisms in the promoter region of the CAPN1 gene in broilers and to investigate their
impact on CAPN1 transcript abundance in breast muscles. The experiment used broilers of two
genetic lines, fast- and slow-growing. Five new polymorphisms in the promoter region of the CAPN1 gene were identified, all of them in linkage disequilibrium (P<0.05). However, the results obtained for their association with expression level were doubtful. Therefore, we surmise that the newly discovered polymorphisms, although they alter the potential sequence binding of transcription factors, probably have just a weak effect on the level of CAPN1 expression in broiler chickens at the investigated stage of ontogenesis.
Keywords :
broilers, CAPN1, polymorphism, promoter region

Date Deposited : 24 Mar 2016 11:24

Last Modified : 24 Mar 2016 11:24

Official URL: http://www.ighz.edu.pl/files/objects/7562/66/str_287-298.pdf

Volume 33, Number 3, June 2015

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