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.: Home > Animal Science Papers and Reports > 2011 > Volume 29 Number 3 > Karima Galal Abdel Hameed1, Gra؟yna Sender2

Prevalence of Helicobacter pullorum in Egyptian hen’s eggs and in vitro susceptibility to different antimicrobial agents

Karima Galal Abdel Hameed1, Gra؟yna Sender2
1 South Valley University, Qena, Egypt 2 Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland
Abstract :

The objective of the study was to investigate the occurrence of H. pullorum in chicken hens’ eggs
and to determine their in vitro susceptibility to various antimicrobial agents. A total of 300 fresh
commercial chicken eggs of Balady native breed and poultry farms (PF) were collected from both
Assiut and Qena Governorates in Egypt. Every five eggs represented one sample (mixed contents of
five eggs). The eggshell and eggshell contents were examined for the presence of Helicobacter species
by conventional methods, next confirmed with PCR. The highest incidence of contamination with
Helicobacter species was recorded in eggs from Assiut Governorate poultry farms. The obtained
results of Helicobacter pullorum (H. pullorum) isolation revealed that poultry farms egg shells and
contents were less contaminated in both Governorates than Balady ones. Additionally, fresh egg
contents of poultry farms in both Governorates were free from H. pullorum. All 12 isolates that were
conventionally classified as carrying H. pullorum occurred free from the organism when tested with
PCR method by detection of one PCR product on agarose gel that matched the predicted size of
477 bp that corresponding to 16S rRNA region of the gene. H. pullorum show in vitro susceptibility
to almost all tested types of antibiotics except for ampicillin, ceftriaxone, and sulphamethoxazole

Keywords :
eggs / Helicobacter pullorum / polymerase chain reaction / antimicrobial susceptibility

Date Deposited : 21 Jun 2011 16:22

Last Modified : 21 Jun 2011 16:27

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Volume 29, Number 3, - 2011

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