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.: Home > Nature and Science > 2011 > Volume 9 Number 2 > Walaa.Hozayen, Mouhamed. Bastawy, Haidy.Elshafeey*

Effects of Aqueous Purslane (Portulaca Oleracea) Extract and Fish Oil on Gentamicin Nephrotoxicity in Albino Rats

Walaa.Hozayen, Mouhamed. Bastawy, Haidy.Elshafeey*
Chemistry Department, Faculty of Sciences, Beni-Suef University, Beni-Suef, Egypt*
Abstract :

Nephrotoxicity is of critical concern when selecting new drug candidates during the early stage of drug development. Because of its unique metabolism, the kidney is an important target of the toxicity of drugs, xenobiotics, and oxidative stress. Gentamicin (GM) is an antibiotic induced nephrotoxicity as it induces conspicuous and characteristic changes in lysosomes of proximal tubular cells consistent with the accumulation of polar lipids (myeloid bodies). These changes are preceded and accompanied by signs of tubular dysfunctions or alterations (release of brush border and lysosomal enzymes; decreased reabsorption of filtered proteins.  The effect of gentamicin (80 mg/kg Bw/day) without or with oral administration of aqueous purslane (Portulaca oleracea) extract (400mg/kg BW/day) and fish oil (5mg/kg BW/day) co-treatments for 15 days was evaluated in adult male rats (80-120g).  Plasma urea, uric acid and creatinine levels were assayed. Lipid peroxidation (indexed by MDA) and antioxidants enzymes like GSH, SOD and CAT were assessed. There was a decrease in plasma levels concentration of urea, uric acid and creatinine, In addition to decreasing in activities of GSH, SOD and CAT as well as an increasing in MDA concentration in the kidney as a result of gentamicin injection. Co-administration of aqueous purslane extract and fish oil was found to improve the adverse changes in the kidney functions with an increase in antioxidants activities and reduction of peroxidation.We propose that dietary fish oil or purslane extract supplementation may provide a cushion for a prolonged therapeutic option against GM nephropathy without harmful side effects.    

Keywords :
purslane,fish oil,nephrotoxicity and antioxidants

Date Deposited : 30 Mar 2016 14:09

Last Modified : 30 Mar 2016 14:09

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Volume 9, Number 2, - 2011 , ISSN 1545-0740

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