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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2016 > Volume 4 Number 1 > Belay Feyisa 1 , Alemu Lencho 1 , Thangavel Selvaraj* 1 and Gezehegne Getaneh 2

Evaluation of Some Botanicals and Trichoderma harzianum for the Management of Tomato Root-knot Nematode ( Meloidogyne incognita (Kofoid and White) Chit Wood)

Belay Feyisa 1 , Alemu Lencho 1 , Thangavel Selvaraj* 1 and Gezehegne Getaneh 2
1 Department of Plant Sciences, College of Agriculture and Veterinary Sciences, Ambo University, Ambo, Post Box No: 19, Ethiopia, East Africa 2 Addis Ababa University, Salale Campus, Addis Ababa, P. B. No: 2003, Ethiopia
Abstract :
Root-knot nematode disease caused  y Meloidogyne incognita (Kofoid and White) Chit wood ) is one of the major constraints for successful cultivation of tomato ( Lycopersicon esculentum Mill.) in Ethiopia. Hence, the present study was conducted to evaluate the effect of leaf and seed extracts of four botanicals viz., Rape seed ( Brassica napus L.) , Lantana ( Lantana camara L .), African marigold (Tagetes erecta L .) and Neem (Azadirachta indica L.) at 5% and 10% concentrations and T. harzianum at 5% plus control were tested on root-knot nematode under in vitro and also to evaluate their against root-knot nematode development and their role on plant growth parameters of tomato under in vivo condition. Plant extracts were more effective and significantly inhibited egg hatching and immobilizing the J 2 larval mortality of M. incognita than T. harzianum.
Aqueous extract of all the tested plants inhibited egg hatching of nematode and resulted84.67-100% mortality of the J 2 juveniles of M. incognita in vitro at the 10% concentration after 72 h of exposure time. There were no significant differences among the treatments of rape seed leaf (84.7%) at 10% concentration and Lantana camara (87%), African marigold (86.3%) and Neem leaf (85%) at 5% concentration after 72 h. Aqueous seed extracts of A. indica more significantly inhibited egg hatching and larva mortality of the J 2 of M. incognita in vitro at the 10% concentration and immobilized by 89, 93 and 100% after 24, 48 and 72 h of exposures, respectively, while at similar concentration of T. erecta, B. napus and L. camara leaf extracts exhibited 92, 89 and 93.2% inhibition of egg hatching and 75, 62.1 and 73% larval mortality, respectively. The effect of different botanicals and T. harzianum singly and in combination were studied for the management of tomato root-knot nematode under greenhouse condition. There was a significant difference in the reduction of root-knot nematode incidence, root-knot nematode population, nematode reproduction rate (NRR), number of galls and egg masses per plant were recorded.
In pot culture condition, the application of leaf extract of individual plant in the presence of the nematode significantly enhanced the growth of tomato seedlings in comparison to the control. A significant increase in plant height, shoot weight and root weight of the seedlings were observed at the 10% concentration of leaf extracts in comparison to control. There was a significant difference in the reduction of root-knot nematode population, nematode reduction rate, number of galls and egg masses per plant of L. camara combined with T. harzianum . The mean fruit weight and total yield were observed highest in the combination treatment of L. camara combined withT. harzianum. Thisstudy results revealed that the test plants are readily available to farmers at no cost and able to reduce nematodepopulation below economic threshold.

 


Keywords :
Tomato seedlings; Botanical leaf; Seed extracts; Trichoderma harzianum ; Root-knot nematode; Egg hatching; Larval mortality; Growth

Date Deposited : 30 Mar 2016 15:08

Last Modified : 30 Mar 2016 15:08

Official URL: http://www.esciencecentral.org/journals/evaluation-of-some-botanicals-and-trichoderma-harzianum-for-themanagement-of-tomato-rootknot-nematode-meloidogyne-incognitakofoid-2329-8863-1000201.php?aid=65649

Volume 4, Number 1, February 2016

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