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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2015 > Volume 3 Number 4 > Mathew PieroNgugi1 *, Oduor Richard Okoth1 , Omwoyo Richard Ombori2 , Njagi Joan Murugi3 , Mgutu Allan Jalemba1 and Cheruiyot Richard Chelule2

Plant Regeneration of Kenyan Cassava (Manihot Esculenta Crantz) Genotypes

Mathew PieroNgugi1 *, Oduor Richard Okoth1 , Omwoyo Richard Ombori2 , Njagi Joan Murugi3 , Mgutu Allan Jalemba1 and Cheruiyot Richard Chelule2
1Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya 2Department of Plant and Microbial Sciences, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya 3Department of Environmental Health, Kenyatta University, P.O Box 437844-00100, Nairobi, Kenya
Abstract :

A reproducible regeneration system based on direct somatic embryogenesis is described for Kenya cassava lines. Cassava plants were regenerated at high frequency by inducing shoot primordial on explants derived from cotyledons of cassava somatic embryos. Various parameters were evaluated on their effects on callus induction, somatic embryogenesis, maturation and germination of somatic embryos as well as recovery of regenerated plantlets. Immature leaf lobes were used as explants for somatic embryogenesis. Three Kenyan cassava genotypes viz; Adhiambo Lera, Kibanda Meno and Serere along with a model cultivar, TMS 60444 were used this system. Remarkable regeneration frequencies were observed in all the evaluated genotypes with Adhiambo Lera showing the best responses. As a result, a highly efficient plant regeneration protocol via germination of somatic embryos was achieved. This system enriches the scope of in vitro regeneration protocols for cassava and is envisaged to be a reliable prerequisite to genetic transformation of African cassava genotypes.

Keywords :
Somatic embryogenesis; Immature; leaf lobes; Kenyan cassava genotypes; Regeneration

Date Deposited : 02 Apr 2016 11:57

Last Modified : 04 Apr 2016 11:35

Official URL: http://1Ethiopian Sugar Corporation Research and Training P.o.box 15, Wonji, Ethiopia 2Bayer Trade Representative Office P.o.box 1/1065, Addis Ababa, Ethiopia

Volume 3, Number 4, November 2015 , ISSN ISSN: 2329-8863

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