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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2015 > Volume 3 Number 3 > Sonia Plaza-Wüthrich1 , Regula Blösch1 and Zerihun Tadele1,2*

Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]

Sonia Plaza-Wüthrich1 , Regula Blösch1 and Zerihun Tadele1,2*
1Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland 2Institute of Biotechnology, Addis Ababa University, P.O. Box 32853, Addis Ababa, Ethiopia
Abstract :

Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in the Horn of Africa particularly in Ethiopia where it is staple food for about 50 million people. Its resilience to extreme environmental conditions and high in nutrition makes tef the preferred crop among both farmers and consumers. The efficiency of in vitro regeneration plays significant role in the improvement of crops. We investigated the efficiency of regeneration in 18 tef genotypes (15 landraces and three improved varieties) using three sizes of immature embryos (small, intermediate and large) as an explant. In vitro regeneration was significantly affected by the genotype and the size of the immature embryo used as a donor. Intermediate-size immature embryos which were 101-350 µm long led to the highest percentage of regeneration. Interestingly, the three improved varieties presented very low regeneration efficiencies whereas the landrace Manyi resulted in consistently superior percentage of in vitro regeneration from all three sizes of explants. The findings of this work provide useful insight into the tef germplasm amenable for the regeneration technique which has direct application in techniques such as transformation. It also signifies the importance of using tef landraces instead of improved varieties for in vitro regeneration.

Keywords :
Eragrostis tef; Immature embryo; Plantlet; Regeneration; Embryogenesis; tef

Date Deposited : 04 Apr 2016 10:49

Last Modified : 04 Apr 2016 10:49

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Volume 3, Number 3, August 2015 , ISSN ISSN: 2329-8863

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