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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 4 > Jiang P*, Chen Y and Wilde HD 1

Optimization of EMS Mutagenesis on Petunia for TILLING

Jiang P*, Chen Y and Wilde HD 1
Horticulture Department, University of Georgia, Athens, Georgia, USA
Abstract :

The development of a chemically-mutagenized population of Petunia hybrida could enable the identification of novel alleles for crop improvement. Conditions were determined for mutagenizing petunia with ethyl methanesulfonate (EMS), while minimizing deleterious effects on viability and fertility. A mutagenized population of the doubled haploid P.  hybrida   line  ‘Mitchell  Diploid’  was  developed  as  a  resource  for  TILLING.  Three  mutagenesis  parameters  were  investigated in this study: the imbibition of seeds prior to EMS treatment, the EMS concentration, and EMS exposure time. Based on these results, the mutagenesis of 2000 petunia seeds for TILLING was conducted with a 12 hour imbibition followed by exposure to 0.1% EMS for 12 hours.

Keywords :
Mutation breeding; Ethyl methanesulfonate; TILLING; Petunia

Date Deposited : 05 Apr 2016 15:14

Last Modified : 05 Apr 2016 15:14

Official URL: http://www.esciencecentral.org/journals/archive-advances-in-crop-science-and-technology-open-access.php

Volume 2, Number 4, November 2014 , ISSN ISSN: 2329-8863

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