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Advances in Crop Science and Technology

.: Home > Advances in Crop Science and Technology > 2014 > Volume 2 Number 4 > Dereje Shimelis 1* , Kassahun Bantte 2 and Tilaye Feyissa 3

Interaction Effects of 6-Benzylaminopurine and Kinetin on In vitro Shoot Multiplication of Two Sugarcane ( Saccharum officinarum L. ) Genotypes

Dereje Shimelis 1* , Kassahun Bantte 2 and Tilaye Feyissa 3
1 Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, P. O. box 15, Wonji, Ethiopia 2 Jimma University College of Agriculture and Veterinary Medicine, Jimma, Ethiopia 3 Addis Abeba University, Science and Technology faculty, Addis Abeba, Ethiopia
Abstract :

In Ethiopia, sugarcane is grown as an important cash and industrial crop. It is not an ideal crop for conventional breeding and it lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize the optimum concentration of 6-benzylaminopurine (0.0, 0. 5, 1.0, 1.5, 2.0, 2.5, and 3.0 mgL-l) and Kinetin (0.0, 0.1, 0.5, and 1.0 mgL-l) combination for shoot multiplication of  C86-12  and  C86-56  genotypes  in  completely  randomized  design  with  5x4x2  factorial  treatment  combinations  arrangements.  The  analysis  of  variance  showed  that  the  interaction  effects  of  BAP,  kinetin  and  genotypes  on the number of shoots per explant, number of leaves per shoot and average shoot length were highly significant (p<0.001). Murashige and Skoog (MS) medium supplemented with 1.5 mgL-l BAP and 0.5 mgL-1 of Kin for B86-12; and 1.5 mgL-1 of BAP and 1.0 mgL-1 of Kin for C86-56 were found to be the optimum media for shoot multiplication. B86-12 showed 33.8 ± 0.837 number of shoots per explant with 13.04 ± 0.089 average number of leaves per shoot and 8.4 ± 0.008 cm shoot length whereas C86-56; 25.6 ± 0.548 number of shoots per explant with average number of leaves per shoot of 9.8 ± 0.447 and shoot length 8.65 ± 0.72 cm was obtained after 30 days of sub culturing. Thus, the optimized protocol can be used for rapid multiplication of the planting materials for commercializing the newly released genotypes within a short period of time.



Keywords :
Sugarcane; BAP; Kin; Shoot multiplication

Date Deposited : 05 Apr 2016 15:20

Last Modified : 05 Apr 2016 15:20

Official URL: http://www.esciencecentral.org/journals/archive-advances-in-crop-science-and-technology-open-access.php

Volume 2, Number 4, November 2014 , ISSN ISSN: 2329-8863

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